General Information

    • DRAMP ID

    • DRAMP03097

    • Peptide Name

    • Drosocin (Glycopeptide,insects, arthropods, invertebrates, animals)

    • Source

    • Drosophila melanogaster (Fruit fly)

    • Family

    • Belongs to the drosocin family

    • Gene

    • Dro

    • Sequence

    • GKPRPYSPRPTSHPRPIRV

    • Sequence Length

    • 19

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Beta turns

    • Structure Description

    • The NMR data clearly indicate a difference between the glycosylated and nonglycosylated forms, i.e., in the perturbation of a turn directly adjacent to the glycosylation site.

    • Helical Wheel Diagram

    • DRAMP03097 helical wheel diagram

    • PDB ID

    • 4EZR resolved by X-ray.

    • Predicted Structure

    • There is no predicted structure for DRAMP03097.

Physicochemical Information

    • Formula

    • C98H160N34O24

    • Absent Amino Acids

    • ACDEFLMNQW

    • Common Amino Acids

    • P

    • Mass

    • 2198.56

    • PI

    • 12.01

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 2

    • Net Charge

    • +6

    • Boman Index

    • -69.5

    • Hydrophobicity

    • -1.579

    • Aliphatic Index

    • 35.79

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 82.78

    • Polar Residues

    • 5

DRAMP03097

DRAMP03097 chydropathy plot

Comments Information

    • Function

    • Antibacterial peptide with strong anti-Gram-negative bacteria activity.

    • PTM

    • O-glycosylation is essential for full biological activity.

    • Tissue specificity

    • In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks.

    • Developmental stage

    • Expressed in larvae and in adults.

    • Induction

    • By bacterial infection.

Literature Information

  • ·Literature 1

    • Title

    • Conformational studies by NMR of the antimicrobial peptide, drosocin, and its non-glycosylated derivative: effects of glycosylation on solution conformation.

    • Reference

    • Biochemistry. 1999 Jan 12;38(2):705-14.

    • Author

    • McManus AM, Otvos L Jr, Hoffmann R, Craik DJ.
  • ·Literature 2

    • Title

    • A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution.

    • Reference

    • J Biol Chem. 1993 Jul 15;268(20):14893-7.

    • Author

    • Bulet P, Dimarcq JL, Hetru C, Lagueux M, Charlet M, Hegy G, Van Dorsselaer A, Hoffmann JA.
  • ·Literature 3

    • Title

    • Isolation from an ant Myrmecia gulosa of two inducible O-glycosylated proline-rich antibacterial peptides.

    • Reference

    • J Biol Chem. 1998 Mar 13;273(11):6139-6143.

    • Author

    • Mackintosh JA, Veal DA, Beattie AJ, Gooley AA.