• • DRAMP ID

  • DRAMP03104

• • Peptide Name

  • Sapecin (defensins; Insects, animals)

• • Source

  • Sarcophaga peregrina (Flesh fly)

• • Family

  • Belongs to the invertebrate defensin family (Type 1 subfamily)

• • Gene

  • Not found

• • Sequence

  • ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKAVCVCRN

• • Sequence Length

  • 40

• • UniProt Entry

  • P18313

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • Gram-positive bacteria: Staphylococcus aureus FDA209P (MIC=5.0 µg/ml), Staphylococcus smith (MIC=5.0 µg/ml), Micrococcus luteus FDA16 (MIC=1.2 µg/ml), Micrococcus luteus IF03333 (MIC=1.2 µg/ml), Micrococcus luteus PC11001 (MIC=10.0 µg/ml), Bacillus subtilis NRRI B-558 (MIC=39.0 µg/ml), Bacillus subtilis PC1219 (MIC=20.0 µg/ml), Mycobacterium smegmatis ATCC607 (MIC=78.0 µg/ml), Corynebacterium bouis 1810 (MIC=2.5 µg/ml), Corynebacterium michiganense (MIC=2.5 µg/ml).
    • Gram-negative bacteria: Xantbmonas oryzae (MIC=20.0 µg/ml), Pseudomonas lachrymans (MIC=78.0 µg/ml), Escherichia coli NIHJ (MIC>78.0 µg/ml), Shigella sonnei JS11746 (MIC>78.0 µg/ml), Proteus vulgaris O X19 (MIC>78.0 µg/ml)

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bonds between Cys3 and Cys30; Cys16 and Cys36; Cys20 and Cys38.

• • Stereochemistry

  • L

• • Structure

  • Combine helix and strand structure

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1L4V resolved by NMR.
• 1L4V-> 

• Predicted Structure

• There is no predicted structure for DRAMP03104.

DRAMP03104

• • Function

  • Sapecins, which are potent bactericidal proteins, are produced in response to injury. Sapecin is cytotoxic to Gram-positive bacteria, and to a lesser extent against Gram-negative bacteria.

• • Tissue specificity

  • Hemocytes and fat body. Induction

• • PTM

  • Contains three disulfide bonds 3-30; 16-36; 20-38.

• ·Literature 1

• • Title

  • Molecular cloning of cDNA for sapecin and unique expression of the sapecin gene during the development of Sarcophaga peregrina.

• • Pubmed ID

  • 3182836

• • Reference

  • J Biol Chem. 1988 Nov 15;263(32):17117-17121.

• • Author

  • Matsuyama K, Natori S.

• ·Literature 2

• • Title

  • Purification of three antibacterial proteins from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.

• • Pubmed ID

  • 3182836

• • Reference

  • J Biol Chem. 1988 Nov 15;263(32):17112-17116.

• • Author

  • Matsuyama K, Natori S.

• ·Literature 3

• • Title

  • Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly).

• • Pubmed ID

  • 2358424

• • Reference

  • J Biochem. 1990 Apr;107(4):514-518.

• • Author

  • Kuzuhara T, Nakajima Y, Matsuyama K, Natori S.