General Information
-
DRAMP ID
- DRAMP03125
-
Peptide Name
- Def-DAA (hybrid defensins; Insects, animals)
-
Source
- Anopheles gambiae (African malaria mosquito)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- ATCDLASKWNWNHTLCAAHCIARRYRGGYCNSKAVCVCR
-
Sequence Length
- 39
-
UniProt Entry
- Q17027
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
-
Target Organism
-
- Gram-positive bacteria: Staphylococcus aureus strain 21 (IC90=0.75 µg/mL), Staphylococcus aureus strain 4 (IC90=1.5 µg/mL).
- T1=8 mg/kg, T2=30 mg/kg.
- T1, dose causing the first signs of toxicity on mice. T2, lethal dose.
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Combine helix and strand structure
-
Structure Description
- Global fold has a N-terminal loop L1, followed by a helix and a two-stranded antiparallel β-sheet forming the CSab motif.
-
Helical Wheel Diagram
-
PDB ID
- 2E3G resolved by NMR.
-
Predicted Structure
- There is no predicted structure for DRAMP03125.
Physicochemical Information
-
Formula
- C186H290N62O51S6
Absent Amino Acids
- EFMPQ
Common Amino Acids
- AC
Mass
- 4403.09
PI
- 9.15
Basic Residues
- 8
Acidic Residues
- 1
Hydrophobic Residues
- 13
Net Charge
- +7
-
Boman Index
- -73.04
Hydrophobicity
- -0.208
Aliphatic Index
- 60.26
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 14355
Absorbance 280nm
- 377.76
Polar Residues
- 17
DRAMP03125
Comments Information
Function
- Among the five hybrid defensins tested, Def-DAA appears to be more efficient than Def-AAA on the multi resistant and sensitive strains.
Literature Information
- ·Literature 1
-
Title
- Rational design of peptides active against the gram positive bacteria Staphylococcus aureus.
-
Pubmed ID
- 18214975
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Reference
- Proteins. 2008 Jul;72(1):229-239.
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Author
- Landon C, Barbault F, Legrain M, Guenneugues M, Vovelle F.