• • DRAMP ID

  • DRAMP03140

• • Peptide Name

  • Anopheles cecropin-A amidated isoform (Insects, animals)

• • Source

  • Anopheles gambiae (African malaria mosquito)

• • Family

  • Belongs to the cecropin family

• • Gene

  • CecA

• • Sequence

  • GRLKKLGKKIEGAGKRVFKAAEKALPVVAGVKALG

• • Sequence Length

  • 35

• • UniProt Entry

  • P82290,A7XAX5,A7XAX7,A7XAY1,A7XAY8,A7XAZ7,A7XB12,A7XB23,Q7QEE3

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • Gram-negative bacteria: Alcaligenes faecalis (MIC=1-2.5 µM), Escherichia coli 1106 (MIC=0.5-1 µM), E. coli D22 (MIC=0.1-0.25 µM), E. coli D31 (MIC=0.1-0.25 µM), E. coli SBS363 (MIC=0.05-0.1 µM), Enterobacter cloacae β12 (MIC=0.5-1 µM), Erwinia carotovora (MIC=0.5-1 µM), Klebsiella pneumoniae (MIC=1-2.5 µM), Pseudomonas aeruginosa (MIC=1-2.5 µM), Salmonella typhimurium (MIC=0.1-0.25 µM), Xanthomonas campestris (MIC=0.5-1 µM);
    • Gram-positive bacteria: Aerococcus viridans (MIC=0.5-1 µM), Bacillus cereus (MIC=50-100 µM), Bacillus megaterium (MIC=0.5-1 µM), Listeria monocytogenes (MIC=50-100 µM), Micrococcus luteus (MIC=0.5-1 µM), Staphylococcus aureus (MIC=50-100 µM), Staphylococcus epidermidis (MIC=10-25 µM), Staphylococcus haemolyticus (MIC=1-2.5 µM), Staphylococcus saprophyticus (MIC=1-2.5 µM), Streptococcus pyogenes (MIC=0.1-0.25 µM).
    • Fungi: Aspergillus fumigatus (MIC=5-10 µM), Botrytis cinerea (MIC=1-2.5 µM), Fusarium culmorum (MIC=0.5-1 µM), Fusarium oxysporum (MIC=1-2.5 µM), Neurospora crassa (MIC=1-2.5 µM), Candida albicans (MIC=1-2.5 µM), Cryptococcus neoformans (MIC=5-10 µM), Saccharomyces cerevisiae (MIC=25-50 µM).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Linear

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP03140_predicted_structure.pdb to download.

DRAMP03140

• • Function

  • Antibacterial activity against several Gram-positive and Gram-negative bacteria. Also has antifungal activity.

• • Tissue specificity

  • Relatively abundant in head, thorax and to a lesser extent in abdominal carcass and anterior midgut.

• • Developmental stage

  • In young pupae, expressed within 2 hours of infection and continued to accumulate over 16 hours.

• • Induction

  • By bacterial infection.

• • PTM

  • C-terminal amidation.

• ·Literature 1

• • Title

  • Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae.

• • Pubmed ID

  • 10672074

• • Reference

  • Insect Mol Biol. 2000 Feb;9(1):75-84.

• • Author

  • Vizioli J, Bulet P, Charlet M, Lowenberger C, Blass C, Müller HM, Dimopoulos G, Hoffmann J, Kafatos FC, Richman A.

• ·Literature 2

• • Title

  • Genomic organization and regulation of three cecropin genes in Anopheles gambiae.

• • Pubmed ID

  • 12421409

• • Reference

  • Insect Mol Biol. 2002 Dec;11(6):517-525.

• • Author

  • Zheng XL, Zheng AL.