General Information

    • DRAMP ID

    • DRAMP03162

    • Peptide Name

    • Phlebotomus duboscqi defensin (PduDef; defensins; Insects, animals)

    • Source

    • Phlebotomus duboscqi

    • Family

    • Belongs to the invertebrate defensin family (Type 1 subfamily)

    • Gene

    • Not found

    • Sequence

    • ATCDLLSAFGVGHAACAAHCIGHGYRGGYCNSKAVCTCRR

    • Sequence Length

    • 40

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal, Antiparasitic

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus (MIC=0.78-1.56 µM), Aspergillus fumigatus (MIC=12.5-25 µM).
      • Fungi: Fusarium culmorum (MIC=1.56-3.12 µM), Fusarium oxysporum (MIC=3.12-6.25 µM), Neurospora crassa (MIC=6.25-12.5 µM), Trichoderma viride (MIC=3.12-6.25 µM), Trichophyton mentagrophytes (MIC=25-50 µM), Candida albicans (MIC=6.25-12.5 µM), Saccharomyces cerevisiae (MIC=25-50 µM).
      • Parasite: Leishmania major promastigote forms (IC50=68-85 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys3 and Cys30; Cys16 and Cys36; Cys20 and Cys36.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03162 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03162.

Physicochemical Information

    • Formula

    • C170H267N57O50S6

    • Absent Amino Acids

    • EMPQW

    • Common Amino Acids

    • A

    • Mass

    • 4101.7

    • PI

    • 8.68

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +6

    • Boman Index

    • -40.06

    • Hydrophobicity

    • 0.223

    • Aliphatic Index

    • 61.25

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 86.03

    • Polar Residues

    • 19

DRAMP03162

DRAMP03162 chydropathy plot

Comments Information

    • Function

    • Has antiparasitic and antifungal activity.

    • Tissue specificity

    • Is synthesized by the fat body and eventually secreted into the hemolymph.

    • Induction

    • By bacterial and parasitic hemolymph and gut infections. Expression peaks at 24 hours post infection by bacterium P. carotovorum, and at day four post infection by the parasite L. major.

    • PTM

    • Problely contains three disulfide bonds 3-30; 16-36; 20-36.

Literature Information

  • ·Literature 1

    • Title

    • Characterization of a defensin from the sand fly Phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite Leishmania major.

    • Reference

    • Infect Immun. 2004 Dec;72(12):7140-7146.

    • Author

    • Boulanger N, Lowenberger C, Volf P, Ursic R, Sigutova L, Sabatier L, Svobodova M, Beverley SM, Späth G, Brun R, Pesson B, Bulet P.