• DRAMP ID

    • DRAMP03162
    • Peptide Name

    • Phlebotomus duboscqi defensin (PduDef; defensins; Insects, animals)
    • Source

    • Phlebotomus duboscqi
    • Family

    • Belongs to the invertebrate defensin family (Type 1 subfamily)
    • Gene

    • Unknown
    • Sequence

    • ATCDLLSAFGVGHAACAAHCIGHGYRGGYCNSKAVCTCRR
    • Sequence Length

    • 40
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Antifungal, Antiparasitic, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus (MIC=0.78-1.56 µM), Aspergillus fumigatus (MIC=12.5-25 µM).
      • Fungi: Fusarium culmorum (MIC=1.56-3.12 µM), Fusarium oxysporum (MIC=3.12-6.25 µM), Neurospora crassa (MIC=6.25-12.5 µM), Trichoderma viride (MIC=3.12-6.25 µM), Trichophyton mentagrophytes (MIC=25-50 µM), Candida albicans (MIC=6.25-12.5 µM), Saccharomyces cerevisiae (MIC=25-50 µM).
      • Parasite: Leishmania major promastigote forms (IC50=68-85 µM).
    • Hemolytic activity

    • Unknown
    • Binding Target

    • Unknown
    • Structure

    • Bridge
    • Structure Description

    • Unknown
    • DRAMP03162 helical wheel diagram
    • Formula

    • C170H267N57O50S6
    • Absent Amino Acids

    • EMPQW
    • Common Amino Acids

    • A
    • Mass

    • 4797.57
    • PI

    • 8.39
    • Basic Residues

    • 7
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 13
    • Boman Index

    • -40.06
    • Hydrophobicity

    • 22.25
    • Aliphatic Index

    • 61.25
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 3355
    • Absorbance 280nm

    • 86.03
    • Polar Residues

    • 19

DRAMP03162

DRAMP03162 chydropathy plot
    • Function

    • Has antiparasitic and antifungal activity.
    • Tissue specificity

    • Is synthesized by the fat body and eventually secreted into the hemolymph.
    • Induction

    • By bacterial and parasitic hemolymph and gut infections. Expression peaks at 24 hours post infection by bacterium P. carotovorum, and at day four post infection by the parasite L. major.
    • PTM

    • Problely contains three disulfide bonds 3-30; 16-36; 20-36.
  • Literature 1
    • Reference

    • Infect Immun. 2004 Dec;72(12):7140-7146.
    • Author

    • Boulanger N, Lowenberger C, Volf P, Ursic R, Sigutova L, Sabatier L, Svobodova M, Beverley SM, Späth G, Brun R, Pesson B, Bulet P.
    • Title

    • Characterization of a defensin from the sand fly Phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite Leishmania major.