General Information

    • DRAMP ID

    • DRAMP03181

    • Peptide Name

    • Spinigerin (Insects, animals)

    • Source

    • Pseudacanthotermes spiniger (Termite)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • HVDKKVADKVLLLKQLRIMRLLTRL

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria: Bacillus megaterium (MIC=0.8-1.5 µM), Micrococcus luteus (MIC=0.8-1.5 µM);
      • Gram-negative bacteria: Escherichia coli SBS363 (MIC=3-6 µM), E. coli D22 (MIC=3-6 µM), Klebsiella pneumoniae (MIC=50-100 µM), Salmonella typhimurium (MIC=3-6 µM), Pseudomonas aeruginosa (MIC=50-100 µM).
      • Fungi: Trichoderma viride (MIC=1.5-3 µM), Neurospora crassa (MIC=3-6 µM), Fusarium culmorum (MIC=1.5-3 µM), Nectria haematococca (MIC=0.4-0.8 µM), Candida albicans (MIC=3-6 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03181 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP03181.

Physicochemical Information

    • Formula

    • C136H247N41O32S

    • Absent Amino Acids

    • CEFGNPSWY

    • Common Amino Acids

    • L

    • Mass

    • 3000.77

    • PI

    • 11.07

    • Basic Residues

    • 8

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +6

    • Boman Index

    • -41.53

    • Hydrophobicity

    • 0.156

    • Aliphatic Index

    • 163.6

    • Half Life

      • Mammalian:3.5 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP03181

DRAMP03181 chydropathy plot

Comments Information

    • Function

    • Active against Gram-positive bacteria and Gram-negative bacteria and fungi.

Literature Information

  • ·Literature 1

    • Title

    • Insect immunity. Constitutive expression of a cysteine-rich antifungal and a linear antibacterial peptide in a termite insect.

    • Reference

    • J Biol Chem. 2001 Feb 9;276(6):4085-4092.

    • Author

    • Lamberty M, Zachary D, Lanot R, Bordereau C, Robert A, Hoffmann JA, Bulet P.
  • ·Literature 2

    • Title

    • Solution structures of stomoxyn and spinigerin, two insect antimicrobial peptides with an alpha-helical conformation.

    • Reference

    • Biopolymers. 2006 Feb 5;81(2):92-103.

    • Author

    • Landon C, Meudal H, Boulanger N, Bulet P, Vovelle F.