General Information
-
DRAMP ID
- DRAMP03183
-
Peptide Name
- Naegleriapore A
-
Source
- Naegleria fowleri (Brain eating amoeba)
-
Family
- Not found
-
Gene
- NP-A
-
Sequence
- DAECEICKFVIQQVEAFIESNHSQAEIQKELNKL
-
Sequence Length
- 34
-
UniProt Entry
- Q95X03
-
Protein Existence
- Predicted
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiprotozoal, Cytotoxic
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Target Organism
-
- Gram-negative bacterium: Escherichia coli K-12 D31;
- Gram-positive bacterium: Bacillus subtilis strain 60015.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Alpha helix
-
Structure Description
- It is characterized by a structure of amphipathic α-helices and an invariant framework of cysteine residues involved in disulfide bonds.
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP03183.
Physicochemical Information
-
Formula
- C171H273N45O57S2
Absent Amino Acids
- GMPRTWY
Common Amino Acids
- E
Mass
- 3935.44
PI
- 4.54
Basic Residues
- 4
Acidic Residues
- 7
Hydrophobic Residues
- 13
Net Charge
- -3
-
Boman Index
- -61.58
Hydrophobicity
- -0.353
Aliphatic Index
- 94.71
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 3.79
Polar Residues
- 6
DRAMP03183
Comments Information
Function
- Posesses antibacterial, cytotoxicity and pore-forming activity. In concentrations up to 10 µm, naegleriapore A is inactive against the Gram-negative bacterium E. coli D31. The specific pore-forming activities of naegleriapore A and B are 3.1±1.7 units pmol−1 and 3.7±1.3 units pmol−1, respectively.
Literature Information
- ·Literature 1
-
Title
- Pore-forming polypeptides of the pathogenic protozoon Naegleria fowleri.
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Pubmed ID
- 11948186
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Reference
- J Biol Chem. 2002 Jun 21;277(25):22353-22360.
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Author
- Herbst R, Ott C, Jacobs T, Marti T, Marciano-Cabral F, Leippe M.