General Information

    • DRAMP ID

    • DRAMP03183

    • Peptide Name

    • Naegleriapore A

    • Source

    • Naegleria fowleri (Brain eating amoeba)

    • Family

    • Not found

    • Gene

    • NP-A

    • Sequence

    • DAECEICKFVIQQVEAFIESNHSQAEIQKELNKL

    • Sequence Length

    • 34

    • Protein Existence

    • Predicted

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiprotozoal, Cytotoxic

    • Target Organism

      • Gram-negative bacterium: Escherichia coli K-12 D31;
      • Gram-positive bacterium: Bacillus subtilis strain 60015.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • It is characterized by a structure of amphipathic α-helices and an invariant framework of cysteine residues involved in disulfide bonds.

    • Helical Wheel Diagram

    • DRAMP03183 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03183.

Physicochemical Information

    • Formula

    • C171H273N45O57S2

    • Absent Amino Acids

    • GMPRTWY

    • Common Amino Acids

    • E

    • Mass

    • 3935.44

    • PI

    • 4.54

    • Basic Residues

    • 4

    • Acidic Residues

    • 7

    • Hydrophobic Residues

    • 13

    • Net Charge

    • -3

    • Boman Index

    • -61.58

    • Hydrophobicity

    • -0.353

    • Aliphatic Index

    • 94.71

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.79

    • Polar Residues

    • 6

DRAMP03183

DRAMP03183 chydropathy plot

Comments Information

    • Function

    • Posesses antibacterial, cytotoxicity and pore-forming activity. In concentrations up to 10 µm, naegleriapore A is inactive against the Gram-negative bacterium E. coli D31. The specific pore-forming activities of naegleriapore A and B are 3.1±1.7 units pmol−1 and 3.7±1.3 units pmol−1, respectively.

Literature Information

  • ·Literature 1

    • Title

    • Pore-forming polypeptides of the pathogenic protozoon Naegleria fowleri.

    • Reference

    • J Biol Chem. 2002 Jun 21;277(25):22353-22360.

    • Author

    • Herbst R, Ott C, Jacobs T, Marti T, Marciano-Cabral F, Leippe M.