• • DRAMP ID

  • DRAMP03215

• • Peptide Name

  • Gomesin (Gm; spiders, Arthropods, animals)

• • Source

  • Acanthoscurria gomesiana (Tarantula spider)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • ZCRRLCYKQRCVTYCRGR

• • Sequence Length

  • 18

• • UniProt Entry

  • P82358,Q86RA2

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor

• • Target Organism

  • • [Ref.10942757]Gram-positive bacteria: Aerococcus viridans (MIC=0.8-1.6 µM), Bacillus cereus (MIC=6.25-12.5 µM), Bacillus megaterium (MIC=0.2-0.4 µM), Bacillus thuringiensis (MIC=1.6-3.15 µM), Enterococcus faecalis (MIC=6.2-12.5 µM), Listeria monocytogenes (MIC=0.8-1.6 µM), Micrococcus luteus (MIC=0.4-0.8 µM), Pediococcus acidolacrici (MIC=3.15-6.25 µM), Staphylococcus aureus (MIC=1.6-3.15 µM), S. epidermidis (MIC=0.8-1.6 µM), S. haemolyticus (MIC=0.8-1.6 µM), S. saprophyticus (MIC=0.8-1.6 µM), Streptococcus pyogenes (MIC=1.6-3.15 µM), Nocardia asteroides (MIC=1.6-3.15 µM);
    • Gram-negative bacteria: Alcaligenes faecalis (MIC>100 µM), Escherichia coli 1106 (MIC=0.8-1.6 µM), E. coli D22 (MIC=0.4-0.8 µM), E. coli D31 (MIC=0.8-1.6 µM), E. coli SBS363 (MIC=0.4-0.8 µM), Erwinia carolovora carolovora (MIC=3.15-6.25 µM), Enterobacter cloacae beta 12 (MIC=3.15-6.25 µM), Klebsiella pneumoniae (MIC=3.15-6.25 µM), Pseudomonas aeruginosa (MIC=1.6-3.15 µM), Salmonella typhimurium (MIC=0.8-1.6 µM), Xhantomonas campestris pv. orizae (MIC=3.15-6.25 µM).
    • Fungi: Alternaria brassicola (MIC=0.4-0.8 µM), Aspergillus fumigatus (MIC=1.6-3.15 µM), Beauveria bassiana (MIC=12.5-25 µM), Fusarium culmorum (MIC=0.4-0.8 µM), F. oxysporum (MIC=0.4-0.8 µM), Neurospora crassa (MIC=0.4-0.8 µM), Nectria haematococca (MIC=0.2-0.4 µM), Tricoderma viridae (MIC=0.4-0.8 µM), Tricophvton mentagrophytes (MIC=0.8-1.6 µM), C. albicans (MIC=0.15-0.3 µM), C. glabrata (MIC=12.5-25 µM), C. tropicalis (MIC=3.15-6.25 µM), C. neoformans (MIC=0.8-1.6 µM), Saccharomyces cerevisiae (MIC=1.6-3.15 µM).
    • [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=32 μM);
    • Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=4 μM), Klebsiella pneumoniae ATCC 700603 (MIC=32 μM), Acinetobacter baumannii ATCC 19606 (MIC=4-8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=8 μM), Helicobacter pylori ATCC 43504 (MIC>80 μM);
    • Fungi : Candida albicans ATCC 90028 (MIC=8-16 μM), Cryptococcus neoformans ATCC 208821 (MIC=0.5-1 μM)

• • Hemolytic Activity

  • • [Ref.28741926] It has 0% hemolysis at 6 μM , 3% hemolysis at 10 μM , 10% hemolysis at 5 μM , 41.2-53.2% hemolysis at 64 μM , 50% hemolysis at 100 μM against human red blood cells

• • Cytotoxicity

  • • [Ref.28741926] CC50 = 67.0 ± 5.0 μM against CRL-1739, CC50 = 3.7 ± 0.2 μM against MM96L, CC50 = 49.9 ±16.6μM against HFF-1, CC50 =54.1±5.0 μM against HeLa, CC50 = 3.8 ± 0.3 μM against K-562.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • pyroglutamic acid

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys2 and Cys15,Cys6 and Cys11.

• • Stereochemistry

  • L

• • Structure

  • Beta strand

• • Structure Description

  • Gm adopts a well-defined β-hairpin-like struc ture, and the disulfide bonds have been reported to be important for the maintenance of this structure.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1KFP resolved by NMR.

• Predicted Structure

• There is no predicted structure for DRAMP03215.

DRAMP03215

• • [Ref.10942757]Function

  • Gomesin bound to the cell surface of cryptococci neoformans, which resulted in cell death associated with membrane permeabilization (FEMS Microbiol Lett. 2007 Sep;274(2)

• ·Literature 1

• • Title

  • Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family.

• • Pubmed ID

  • 10942757

• • Reference

  • J Biol Chem. 2000 Oct 27;275(43):33464-33470.

• • Author

  • Silva PI Jr, Daffre S, Bulet P.

• ·Literature 2

• • Title

  • Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties

• • Pubmed ID

  • 28741926

• • Reference

  • ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459. 

• • Author

  • Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik