General Information

    • DRAMP ID

    • DRAMP03227

    • Peptide Name

    • M-zodatoxin-Lt2a (M-ZDTX-Lt2a; Latarcin-2a, Ltc-2a, Ltc2a; spiders, Arthropods, animals)

    • Source

    • Lachesana tarabaevi (Spider)

    • Family

    • Belongs to the latarcin family

    • Gene

    • Not found

    • Sequence

    • GLFGKLIKKFGRKAISYAVKKARGKH

    • Sequence Length

    • 26

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.16735513]Gram-positive bacteria: Arthrobacter globiformis VKM Ac-1112 (MIC=0.7 µM), Bacillus subtilis VKM B-501 (MIC=0.4 µM);
      • Gram-negative bacteria: Escherichia coli DH5-alpha (MIC=1.0 µM), Escherichia coli MH1 (MIC=0.7 µM), Pseudomonas aeruginosa PAO1 (MIC=6.7 µM);
      • Fungi: Pichia pastoris GS115 (MIC=6.7 µM), Saccharomyces cerevisiae Y190 (MIC=54 µM).

    • Hemolytic Activity

      • [Ref.16735513]20% hemolytic activity at 6 μM against rabbit erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix (2 helices; 18 residues)

    • Structure Description

    • The molecule of Ltc2a is found to consist of two helical regions (residues 3-9 and 13-21) connected via a poorly ordered fragment.

    • Helical Wheel Diagram

    • DRAMP03227 helical wheel diagram

    • PDB ID

    • 2G9P resolved by NMR.
  • 2G9P-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C136H229N41O29

    • Absent Amino Acids

    • CDEMNPQTW

    • Common Amino Acids

    • K

    • Mass

    • 2902.57

    • PI

    • 11.33

    • Basic Residues

    • 10

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +10

    • Boman Index

    • -38.02

    • Hydrophobicity

    • -0.439

    • Aliphatic Index

    • 82.69

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 59.6

    • Polar Residues

    • 6

DRAMP03227

DRAMP03227 chydropathy plot

Comments Information

    • Function

    • Has hemolytic activity against Rabbit erythrocytes and has toxicity to ovarian insect Sf9 cells (EC50=20 µM). Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.

Literature Information

  • ·Literature 1

    • Title

    • Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity.

    • Reference

    • J Biol Chem. 2006 Jul 28;281(30):20983-20992.

    • Author

    • Kozlov SA, Vassilevski AA, Feofanov AV, Surovoy AY, Karpunin DV, Grishin EV.
  • ·Literature 2

    • Title

    • Spatial structure and activity mechanism of a novel spider antimicrobial peptide.

    • Reference

    • Biochemistry. 2006 Sep 5;45(35):10759-10767.

    • Author

    • Dubovskii PV, Volynsky PE, Polyansky AA, Chupin VV, Efremov RG, Arseniev AS.