General Information
-
DRAMP ID
- DRAMP03231
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Peptide Name
- M-zodatoxin-Lt4a (M-ZDTX-Lt4a; Latarcin-4a, Ltc-4a; spiders, Arthropods, animals)
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Source
- Lachesana tarabaevi (Spider)
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Family
- Belongs to the latarcin family
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Gene
- Not found
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Sequence
- GLKDKFKSMGEKLKQYIQTWKAKF
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Sequence Length
- 24
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UniProt Entry
- Q1ELU5
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Protein Existence
- Protein level
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.16735513]Gram-positive bacteria: Arthrobacter globiformis VKM Ac-1112 (MIC=0.3 µM), Bacillus subtilis VKM B-501 (MIC=1.1 µM);
- Gram-negative bacteria: Escherichia coli DH5-alpha (MIC=4.5 µM), Escherichia coli MH1 (MIC=3.2 µM), Pseudomonas aeruginosa PAO1 (MIC>35 µM);
- Fungi: Pichia pastoris GS115 (MIC=36 µM), Saccharomyces cerevisiae Y190 (MIC=18 µM).
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Hemolytic Activity
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- [Ref.16735513]20% hemolytic activity at >120 μM against rabbit erythrocytes
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- Free
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Stereochemistry
- L
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Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- Please click DRAMP03231_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C136H216N34O34S
Absent Amino Acids
- CHNPRV
Common Amino Acids
- K
Mass
- 2903.48
PI
- 10
Basic Residues
- 7
Acidic Residues
- 2
Hydrophobic Residues
- 7
Net Charge
- +5
-
Boman Index
- -42.48
Hydrophobicity
- -1.017
Aliphatic Index
- 52.92
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 6990
Absorbance 280nm
- 303.91
Polar Residues
- 5
DRAMP03231
Comments Information
Function
- Has hemolytic activity against Rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.
Tissue specificity
- Expressed by the venom gland.
Literature Information
- ·Literature 1
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Title
- Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity.
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Pubmed ID
- 16735513
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Reference
- J Biol Chem. 2006 Jul 28;281(30):20983-209892.
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Author
- Kozlov SA, Vassilevski AA, Feofanov AV, Surovoy AY, Karpunin DV, Grishin EV.