General Information

    • DRAMP ID

    • DRAMP03233

    • Peptide Name

    • M-zodatoxin-Lt5a (M-ZDTX-Lt5a; Latarcin-5, Ltc-5; spiders, Arthropods, animals)

    • Source

    • Lachesana tarabaevi (Spider)

    • Family

    • Belongs to the latarcin family

    • Gene

    • Not found

    • Sequence

    • GFFGKMKEYFKKFGASFKRRFANLKKRL

    • Sequence Length

    • 28

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.16735513]Gram-positive bacteria: Arthrobacter globiformis VKM Ac-1112 (MIC=1.1 µM), Bacillus subtilis VKM B-501 (MIC=0.6 µM);
      • Gram-negative bacteria: Escherichia coli DH5-alpha (MIC=0.6 µM), Escherichia coli MH1 (MIC=0.6 µM), Pseudomonas aeruginosa PAO1 (MIC=18 µM);
      • Fungi: Pichia pastoris GS115 (MIC>37 µM), Saccharomyces cerevisiae Y190 (MIC>37 µM).

    • Hemolytic Activity

      • [Ref.16735513]20% hemolytic activity at 40 μM against rabbit erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03233 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C164H253N45O34S

    • Absent Amino Acids

    • CDHIPQTVW

    • Common Amino Acids

    • K

    • Mass

    • 3431.15

    • PI

    • 11.24

    • Basic Residues

    • 10

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +9

    • Boman Index

    • -64.09

    • Hydrophobicity

    • -0.757

    • Aliphatic Index

    • 35

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 55.19

    • Polar Residues

    • 6

DRAMP03233

DRAMP03233 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity against Gram-positive bacteria and Gram-negative bacteria and and yeasts. Also has hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.

    • Tissue specificity

    • Expressed by the venom gland. PTM

Literature Information

  • ·Literature 1

    • Title

    • Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity.

    • Reference

    • J Biol Chem. 2006 Jul 28;281(30):20983-20992.

    • Author

    • Kozlov SA, Vassilevski AA, Feofanov AV, Surovoy AY, Karpunin DV, Grishin EV.