General Information

    • DRAMP ID

    • DRAMP03253

    • Peptide Name

    • M-lycotoxin-Ls3a (M-LCTX-Ls3a; Lycocitin-1; spiders, Arthropods, animals)

    • Source

    • Lycosa singoriensis (Wolf spider) (Aranea singoriensis)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GKLQAFLAKMKEIAAQTL

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis ATCC6633 (MIC=1.6-3.2 µM);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=3.2-6.4 µM), Pseudomonas aeruginosa.
      • Yeast: Candida albicans.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03253 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03253.

Physicochemical Information

    • Formula

    • C89H153N23O24S

    • Absent Amino Acids

    • CDHNPRSVWY

    • Common Amino Acids

    • A

    • Mass

    • 1961.39

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -3.92

    • Hydrophobicity

    • 0.25

    • Aliphatic Index

    • 108.89

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP03253

DRAMP03253 chydropathy plot

Comments Information

    • Function

    • Inhibits growth of Gram-positive and Gram-negative bacteria and fungi.

    • Tissue specificity

    • Expressed by the venom gland.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • De novo sequencing of antimicrobial peptides isolated from the venom glands of the wolf spider Lycosa singoriensis.

    • Reference

    • J Mass Spectrom. 2004 Feb;39(2):193-201.

    • Author

    • Budnik BA, Olsen JV, Egorov TA, Anisimova VE, Galkina TG, Musolyamov AK, Grishin EV, Zubarev RA.