General Information
-
DRAMP ID
- DRAMP03254
-
Peptide Name
- M-lycotoxin-Ls3b (M-LCTX-Ls3b; Lycocitin-2; spiders, Arthropods, animals)
-
Source
- Lycosa singoriensis (Wolf spider) (Aranea singoriensis)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GRLQAFLAKMKEIAAQTL
-
Sequence Length
- 18
-
UniProt Entry
- P0C2U7
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- Gram-positive bacteria: Staphylococcus aureus 209P, Bacillus subtilis ATCC 6633 (MIC=1.57-3.14 µM);
- Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=3.14-6.29 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=5.02-10.05 µM).
- Fungi: Candida albicans.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- Free
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP03254.
Physicochemical Information
-
Formula
- C89H153N25O24S
Absent Amino Acids
- CDHNPSVWY
Common Amino Acids
- A
Mass
- 1989.41
PI
- 9.99
Basic Residues
- 3
Acidic Residues
- 1
Hydrophobic Residues
- 9
Net Charge
- +2
-
Boman Index
- -13.29
Hydrophobicity
- 0.217
Aliphatic Index
- 108.89
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP03254
Comments Information
Function
- Inhibits growth of Gram-positive and Gram-negative bacteria and fungi.
Tissue specificity
- Expressed by the venom gland.
PTM
- C-terminal amidation.
Literature Information
- ·Literature 1
-
Title
- De novo sequencing of antimicrobial peptides isolated from the venom glands of the wolf spider Lycosa singoriensis.
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Pubmed ID
- 14991689
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Reference
- J Mass Spectrom. 2004 Feb;39(2):193-201.
-
Author
- Budnik BA, Olsen JV, Egorov TA, Anisimova VE, Galkina TG, Musolyamov AK, Grishin EV, Zubarev RA.