General Information

    • DRAMP ID

    • DRAMP03278

    • Peptide Name

    • M-lycotoxin-Hc1a (M-LCTX-Hc1a; Lycotoxin I; spiders, Arthropods, animals)

    • Source

    • Hogna carolinensis (Carolina wolf spider) (Lycosa carolinensis)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IWLTALKFLGKHAAKHLAKQQLSKL

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria: Bacillus thuringiensis subsp. Israelensis (MIC<5.0 µM);
      • Gram-negative bacteria: Escherichia coli D31 (MIC=10-20 µM), Escherichia coli DH5 (MIC=80-150 µM).
      • Fungi: Candida glabrata ATCC 2001 (MIC=100-150 µM), Candida albicans clinical isolate (MIC=100-200 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03278 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03278.

Physicochemical Information

    • Formula

    • C135H223N37O30

    • Absent Amino Acids

    • CDEMNPRVY

    • Common Amino Acids

    • L

    • Mass

    • 2844.49

    • PI

    • 10.6

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +7

    • Boman Index

    • -6.19

    • Hydrophobicity

    • 0.064

    • Aliphatic Index

    • 125.2

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 229.17

    • Polar Residues

    • 3

DRAMP03278

DRAMP03278 chydropathy plot

Comments Information

    • Function

    • Forms pore that permeabilize the cell membrane. Promotes efflux of calcium from synaptosomes, causes hemolysis, and dissipates voltage gradients across muscle membrane. Potently inhibits the growth of bacteria, yeast and Leishmania. Is lethal to lepidopteran larvae. May Function

Literature Information

  • ·Literature 1

    • Title

    • Lycotoxins, antimicrobial peptides from venom of the wolf spider Lycosa carolinensis.

    • Reference

    • J Biol Chem. 1998 Jan 23;273(4):2059-2066.

    • Author

    • Yan L, Adams ME.
  • ·Literature 2

    • Title

    • Membrane structure and interactions of a short Lycotoxin I analogue.

    • Reference

    • J Pept Sci. 2008 Apr;14(4):528-534.

    • Author

    • Adão R, Seixas R, Gomes P, Pessoa JC, Bastos M.