General Information
-
DRAMP ID
- DRAMP03279
-
Peptide Name
- M-lycotoxin-Hc2a (M-LCTX-Hc2a; Lycotoxin-2; Lycotoxin II; spiders, Arthropods, animals)
-
Source
- Hogna carolinensis (Carolina wolf spider) (Lycosa carolinensis)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KIKWFKTMKSIAKFIAKEQMKKHLGGE
-
Sequence Length
- 27
-
UniProt Entry
- P61508
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- Gram-positive bacteria: Bacillus thuringiensis subsp. Israelensis (MIC=10-60 µM);
- Gram-negative bacteria: Escherichia coli D31 (MIC<10 µM), Escherichia coli DH5 (MIC<40 µM).
- Fungi: Candida glabrata ATCC 2001 (MIC=100-150 µM)
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Cell membrane
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- Free
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP03279.
Physicochemical Information
-
Formula
- C149H245N39O35S2
Absent Amino Acids
- CDNPRVY
Common Amino Acids
- K
Mass
- 3206.94
PI
- 10.22
Basic Residues
- 9
Acidic Residues
- 2
Hydrophobic Residues
- 9
Net Charge
- +7
-
Boman Index
- -36.02
Hydrophobicity
- -0.659
Aliphatic Index
- 65.19
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 211.54
Polar Residues
- 4
DRAMP03279
Comments Information
Function
- Forms pore that permeabilize the cell membrane. Promotes efflux of calcium from synaptosomes, causes hemolysis, and dissipates voltage gradients across muscle membrane. Potently inhibits the growth of bacteria, yeast and Leishmania. May Function
Literature Information
- ·Literature 1
-
Title
- Lycotoxins, antimicrobial peptides from venom of the wolf spider Lycosa carolinensis.
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Pubmed ID
- 9442044
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Reference
- J Biol Chem. 1998 Jan 23;273(4):2059-2066.
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Author
- Yan L, Adams ME.
- ·Literature 2
-
Title
- Membrane structure and interactions of a short Lycotoxin I analogue.
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Pubmed ID
- 18098329
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Reference
- J Pept Sci. 2008 Apr;14(4):528-534.
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Author
- Adão R, Seixas R, Gomes P, Pessoa JC, Bastos M.