General Information
-
DRAMP ID
- DRAMP03318
-
Peptide Name
- Pore-forming peptide ameobapore B (EH-APP; saposin-like protein)
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Source
- Entamoeba histolytica (protozoan parasite)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GAILCNLCKDTVKLVENLLTVDGAQAVRQYIDNLCGKASGFLGTLCEKILSFGVDELVKLIENHVDPVVVCEKIHAC
-
Sequence Length
- 77
-
UniProt Entry
- Q24824
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- Gram-positive bacterium: Micrococcus luteus.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Cell membrane
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Not found
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP03318.
Physicochemical Information
-
Formula
- C366H604N96O110S6
Absent Amino Acids
- MW
Common Amino Acids
- LV
Mass
- 8301.76
PI
- 5.14
Basic Residues
- 9
Acidic Residues
- 10
Hydrophobic Residues
- 33
Net Charge
- -1
-
Boman Index
- -40.03
Hydrophobicity
- 0.475
Aliphatic Index
- 125.19
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1865
Absorbance 280nm
- 24.54
Polar Residues
- 22
DRAMP03318
Comments Information
Forms pores in the cytoplasmic membrane of host cells. Implicated in the cytolytic activity of the parasite.
Literature Information
- ·Literature 1
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Title
- Amoebapores, a family of membranolytic peptides from cytoplasmic granules of Entamoeba histolytica: isolation, primary structure, and pore formation in bacterial cytoplasmic membranes.
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Pubmed ID
- 7715451
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Reference
- Mol Microbiol. 1994 Dec;14(5):895-904.
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Author
- Leippe M, Andrä J, Nickel R, Tannich E, Müller-Eberhard HJ.