General Information

    • DRAMP ID

    • DRAMP03322

    • Peptide Name

    • PW2

    • Source

    • Phage display peptide library

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • HPLKQYWWRPSI

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • Fungi: Eimeria acervulina sporozoites, E. tenella sporozoites, Toxoplasma gondii tachyzoites (low activity).

    • Hemolytic Activity

      • [Ref.11849705]1.60% hemolytic activity at 100 μM against human erythrocytes,1.57% hemolytic activity at 100 μM against rabbit erythrocytes,1.42% hemolytic activity at 100 μM against chicken erythrocytes,

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Non helix or strand structure

    • Structure Description

    • In the absence of an interface, PW2 is in random coil conformation. In micelles, structural calculation shows that Trp-7 forms the hydrophobic core that is important for the peptide folding. Lys-4, Tyr-6, Trp-8, and Arg-9 are in the same surface, possibly facing the micelle interface. (Ref.2)

    • Helical Wheel Diagram

    • DRAMP03322 helical wheel diagram

    • PDB ID

    • 1M02 resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP03322.

Physicochemical Information

    • Formula

    • C79H111N21O16

    • Absent Amino Acids

    • ACDEFGMNTV

    • Common Amino Acids

    • PW

    • Mass

    • 1610.88

    • PI

    • 9.99

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +3

    • Boman Index

    • -19.71

    • Hydrophobicity

    • -1.158

    • Aliphatic Index

    • 65

    • Half Life

      • Mammalian:3.5 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 12490

    • Absorbance 280nm

    • 1135.45

    • Polar Residues

    • 2

DRAMP03322

Comments Information

    • Function

    • Synthetic PW2 shows anticoccidial activity against E. acervulina and Eimeria tenella with very low hemolytic activity. It also displays antifungal activity but no activity against bacteria.(Ref.2)

Literature Information

  • ·Literature 1

    • Title

    • Avian anticoccidial activity of a novel membrane-interactive peptide selected from phage display libraries.

    • Reference

    • Mol Biochem Parasitol. 2002 Mar;120(1):53-60.

    • Author

    • da Silva A Jr, Kawazoe U, Freitas FF, Gatti MS, Dolder H, Schumacher RI, Juliano MA, da Silva MJ, Leite A.
  • ·Literature 2

    • Title

    • NMR structure of PW2 bound to SDS micelles. A tryptophan-rich anticoccidial peptide selected from phage display libraries.

    • Reference

    • Biol Chem. 2002 Sep 27;277(39):36351-6.

    • Author

    • co LW, Da Silva A Jr, Leite A, Valente AP, Almeida FC.