General Information

    • DRAMP ID

    • DRAMP03330

    • Peptide Name

    • Alpha-defensin cryptdin-2 (Defensin-related cryptdin-2; Rodents, mammals, animals)

    • Source

    • Mus musculus (Mouse)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • Defa2

    • Sequence

    • LRDLVCYCRTRGCKRRERMNGTCRKGHLMYTLCCR

    • Sequence Length

    • 35

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Listeria monocytogenes EGD;
      • Gram-negative bacteria: Escherichia coli ML-35p, mouse-avirulent Salmonella typhimurium 7953.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys6 and Cys34,Cys8 and Cys23,Cys13 and Cys33.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03330 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03330.

Physicochemical Information

    • Formula

    • C172H296N64O46S8

    • Absent Amino Acids

    • AFIPQSW

    • Common Amino Acids

    • R

    • Mass

    • 4253.12

    • PI

    • 9.86

    • Basic Residues

    • 11

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +9

    • Boman Index

    • -126.36

    • Hydrophobicity

    • -0.72

    • Aliphatic Index

    • 52.86

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 98.68

    • Polar Residues

    • 15

DRAMP03330

DRAMP03330 chydropathy plot

Comments Information

    • Function

    • Has broad-spectrum antibacterial activity. Probably contributes to the antimicrobial barrier Function

    • Tissue specificity

    • Paneth cells of the small bowel.

    • PTM

    • Contains three disulfide bonds 6-34; 8-23; 13-33 (By similarity).

Literature Information

  • ·Literature 1

    • Title

    • Enteric defensins: antibiotic peptide components of intestinal host defense.

    • Reference

    • J Cell Biol. 1992 Aug;118(4):929-936.

    • Author

    • Selsted ME, Miller SI, Henschen AH, Ouellette AJ.
  • ·Literature 2

    • Title

    • Cryptdins: antimicrobial defensins of the murine small intestine.

    • Reference

    • Infect Immun. 1992 Sep;60(9):3556-3565.

    • Author

    • Eisenhauer PB, Harwig SS, Lehrer RI.