General Information

    • DRAMP ID

    • DRAMP03334

    • Peptide Name

    • Alpha-defensin cryptdin-6/12 (Defensin-related cryptdin-6/12; Rodents, mammals, animals)

    • Source

    • Mus musculus (Mouse)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • Defa6 AND Defa12

    • Sequence

    • DLVCYCRARGCKGRERMNGTCRKGHLLYMLCCR

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Listeria monocytogenes EGD;
      • Gram-negative bacteria: Escherichia coli ML-35p, mouse-avirulent Salmonella typhimurium 7953.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys4 and Cys32,Cys6 and Cys21,Cys11 and Cys31.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03334 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03334.

Physicochemical Information

    • Formula

    • C157H266N56O42S8

    • Absent Amino Acids

    • FIPQSW

    • Common Amino Acids

    • CR

    • Mass

    • 3866.67

    • PI

    • 9.38

    • Basic Residues

    • 9

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +7

    • Boman Index

    • -88.63

    • Hydrophobicity

    • -0.406

    • Aliphatic Index

    • 59.09

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 104.84

    • Polar Residues

    • 14

DRAMP03334

DRAMP03334 chydropathy plot

Comments Information

    • Function

    • Has broad-spectrum antibacterial activity. Probably contributes to the antimicrobial barrier Function

    • Tissue specificity

    • Paneth cells of the small bowel.

    • PTM

    • Contains three disulfide bondss 4-32; 6-21; 11-31. Probably contributes to the antimicrobial barrier Function

Literature Information

  • ·Literature 1

    • Title

    • Mouse Paneth cell defensins: primary structures and antibacterial activities of numerous cryptdin isoforms.

    • Reference

    • Infect. Immun. 1994;62:5040-5047.

    • Author

    • Ouellette AJ, Hsieh MM, Nosek MT, Cano-Gauci DF, Huttner KM, Buick RN, Selsted ME.
  • ·Literature 2

    • Title

    • Cryptdins: antimicrobial defensins of the murine small intestine.

    • Reference

    • Infect. Immun. 1992;60:3556-3565.

    • Author

    • Eisenhauer P.B, Harwig S.S.S.L, Lehrer R.I.