General Information

    • DRAMP ID

    • DRAMP03357

    • Peptide Name

    • Cryptdin related sequence peptide (CRS4C-1a; Rodents, mammals, animals)

    • Source

    • Mus musculus (Mouse)

    • Family

    • Not found

    • Gene

    • Defa-rs2

    • Sequence

    • LQDAALGWGRRCPQCPRCPSCPSCPRCPRCPRCKCNPK

    • Sequence Length

    • 38

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Enterococcus faecalis (LC=1.9 µM), Lactobacillus fermentum DSM 20052 (LC=1.1 µM), Strepococcus pyogenes clinical isolate (LC=0.4 µM), Listeria monocytogenes type 1 clinical isolate (LC=1.4 µM);
      • Gram-negative bacteria: Escherichia coli strain D21 (LC=2.4 µM), Salmonella typhimurium ATCC 14028 (LC=1.7 µM).
      • NOTE: Dimerized peptide was two- to fourfold more potent than monomeric peptide. LC, Lethal concentrations.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Lipopolysaccharide (LPS)-binding

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03357 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03357.

Physicochemical Information

    • Formula

    • C172H284N62O46S9

    • Absent Amino Acids

    • EFHIMTVY

    • Common Amino Acids

    • C

    • Mass

    • 4245.07

    • PI

    • 9.24

    • Basic Residues

    • 8

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +7

    • Boman Index

    • -104.67

    • Hydrophobicity

    • -0.821

    • Aliphatic Index

    • 25.79

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 6000

    • Absorbance 280nm

    • 162.16

    • Polar Residues

    • 14

DRAMP03357

DRAMP03357 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide that defense response to Gram-negative bacterium and Gram-positive bacterium.

    • MOA

    • CRS peptides were able to bind LPS and to reduce its immunostimulatory activity, leading to a substantial reduction in cellular activation.

Literature Information

  • ·Literature 1

    • Title

    • Increased diversity of intestinal antimicrobial peptides by covalent dimer formation.

    • Reference

    • Nat Immunol. 2004 Aug;5(8):836-843.

    • Author

    • Hornef MW, Pütsep K, Karlsson J, Refai E, Andersson M.