General Information
-
DRAMP ID
- DRAMP03369
-
Peptide Name
- Beta-defensin 4 (BD-4, mBD-4; Defensin, beta 4; Rodents, mammals, animals)
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Source
- Mus musculus (Mouse)
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Family
- Belongs to the beta-defensin family
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Gene
- Defb4
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Sequence
- QIINNPITCMTNGAICWGPCPTAFRQIGNCGHFKVRCCKIR
-
Sequence Length
- 41
-
UniProt Entry
- P82019
-
Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- Gram-negative bacterium: Escherichia coli;
- Gram-positive bacterium: Staphylococcus aureus.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys9 and Cys37,Cys16 and Cys30,Cys20 and Cys38.
-
Stereochemistry
- L
-
Structure
- Bridge
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP03369.
Physicochemical Information
-
Formula
- C196H315N61O51S7
Absent Amino Acids
- DELSY
Common Amino Acids
- CI
Mass
- 4566.46
PI
- 9.22
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 12
Net Charge
- +6
-
Boman Index
- -46.61
Hydrophobicity
- 0.059
Aliphatic Index
- 69.02
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5875
Absorbance 280nm
- 146.88
Polar Residues
- 17
DRAMP03369
Comments Information
PTM
- Contains three disulfide bonds 9-37; 16-30; 20-38. When expressed as a fusion protein, mBD-4 showed activity against both E. coli and S. aureus at 1ug/ml as well as chemotactic activity via CCR6-expressing cells (Rohrl J et al. J Biol Chem. 2010 Mar 5;285(10)
Literature Information
- ·Literature 1
-
Title
- A novel murine beta-defensin expressed in tongue, esophagus, and trachea.
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Pubmed ID
- 10922379
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Reference
- J Biol Chem. 2000 Oct 27;275(43):33314-33320.
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Author
- Jia HP, Wowk SA, Schutte BC, Lee SK, Vivado A, Tack BF, Bevins CL, McCray PB Jr.