General Information
-
DRAMP ID
- DRAMP03421
-
Peptide Name
- Neutrophil antibiotic peptide NP-3 (RatNP-3a, RatNP-3b; Rodents, mammals, animals)
-
Source
- Rattus norvegicus (Rat)
-
Family
- Belongs to the alpha-defensin family
-
Gene
- Not found
-
Sequence
- CSCRTSSCRFGERLSGACRLNGRIYRLCC
-
Sequence Length
- 29
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiviral
-
Target Organism
-
- Gram-positive bacterium: Staphylococcus aureus 502A;
- Gram-negative bacterium: Escherichia coli ML-35.
- Fungi: Candida albicans 820, Cryptococcus neoformans A-383.
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Cyclization(Cys1 and Cys29).
-
C-terminal Modification
- Cyclization(Cys1 and Cys29).
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys1 and Cys29,Cys3 and Cys18,Cys8 and Cys28.
-
Stereochemistry
- L
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP03421.
Physicochemical Information
-
Formula
- C130H220N48O39S6
Absent Amino Acids
- DHKMPQVW
Common Amino Acids
- CR
Mass
- 3271.83
PI
- 9.25
Basic Residues
- 6
Acidic Residues
- 1
Hydrophobic Residues
- 6
Net Charge
- +5
-
Boman Index
- -84.31
Hydrophobicity
- -0.169
Aliphatic Index
- 57.24
Half Life
-
- Mammalian:1.2 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1865
Absorbance 280nm
- 66.61
Polar Residues
- 16
DRAMP03421
Comments Information
Active in vitro against S. aureus, fungi, Gram-positive and Gram-negative bacteria and to a lesser extent against an enveloped virus.
Literature Information
- ·Literature 1
-
Title
- Polymorphic expression of defensins in neutrophils from outbred rats.
-
Pubmed ID
- 2254017
-
Reference
- Infect. Immun. 1990;58:3899-3902.
-
Author
- Eisenhauer P.B, Harwig S.S.S.L, Szklarek D, Ganz T, Lehrer R.I.
- ·Literature 2
-
Title
- Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis.
-
Pubmed ID
- 7594610
-
Reference
- J. Immunol. 1995;155:4476-4484.
-
Author
- Yount N.Y, Wang MS.C, Yuan J, Banaiee N, Ouellette A.J, Selsted M.E.