General Information

    • DRAMP ID

    • DRAMP03422

    • Peptide Name

    • Neutrophil antibiotic peptide NP-4 (RatNP-4; Rodents, mammals, animals)

    • Source

    • Rattus norvegicus (Rat)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • Np4

    • Sequence

    • ACYCRIGACVSGERLTGACGLNGRIYRLCCR

    • Sequence Length

    • 31

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiviral, Antifungal

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus 502A (MIC=50 µg/ml);
      • Gram-negative bacteria: Escherichia coli ML-35, Acinetobacter calcoaceticus (MIC=50 µg/ml).Fungi: Cryptococcus neoformans (MIC=10 µg/ml), Candida albicans.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys2 and Cys30,Cys4 and Cys19,Cys9 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03422 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03422.

Physicochemical Information

    • Formula

    • C136H229N47O39S6

    • Absent Amino Acids

    • DFHKMPQW

    • Common Amino Acids

    • C

    • Mass

    • 3338.97

    • PI

    • 8.96

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • -47.85

    • Hydrophobicity

    • 0.303

    • Aliphatic Index

    • 81.94

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 111.83

    • Polar Residues

    • 16

DRAMP03422

DRAMP03422 chydropathy plot

Comments Information

    • Active in vitro against S. aureus, fungi, Gram-positive and Gram-negative bacteria and to a lesser extent against an enveloped virus.

Literature Information

  • ·Literature 1

    • Title

    • Purification and antimicrobial properties of three defensins from rat neutrophils.

    • Reference

    • Infect. Immun. 1989;57:2021-2027.

    • Author

    • Eisenhauer P.B, Harwig S.S.S.L, Szklarek D, Ganz T, Selsted M.E, Lehrer R.I.
  • ·Literature 2

    • Title

    • Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis.

    • Reference

    • J. Immunol. 1995;155:4476-4484.

    • Author

    • Yount N.Y, Wang MS.C, Yuan J, Banaiee N, Ouellette A.J, Selsted M.E.