General Information
-
DRAMP ID
- DRAMP03464
-
Peptide Name
- Cryptonin (Insects, animals)
-
Source
- Cryptotympana dubia (Korean horse cicada)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GLLNGLALRLGKRALKKIIKRLCR
-
Sequence Length
- 24
-
UniProt Entry
- P85028
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
-
Target Organism
-
- [Ref.18000874]Gram-positive bacteria: Bacillus subtilis KCTC 3086 (MIC=3.12 µg/ml), Staphylococcus aureus KCTC 1928 (MIC=25 µg/ml), Micrococcus luteus KCTC 3063 (MIC=1.56 µg/ml), Staphylococcus aureus (MRSA) (MIC=25 µg/ml), VRE (MIC=25 µg/ml);
- Gram-negative bacteria: Escherichia coli K12-594 (MIC=3.12 µg/ml);
- Fungi: Candida albicans KCTC 7965 (MIC=50 µg/ml), Candida tropicalis KCTC 1925 (MIC=3.12 µg/ml).
-
Hemolytic Activity
-
- [Ref.18000874]<5% hemolytic activity at 25-200 µg/ml against rat erythrocytes
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- Free
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- Please click DRAMP03464_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C121H227N41O26S
Absent Amino Acids
- DEFHMPQSTVWY
Common Amino Acids
- L
Mass
- 2704.45
PI
- 12.02
Basic Residues
- 8
Acidic Residues
- 0
Hydrophobic Residues
- 11
Net Charge
- +8
-
Boman Index
- -36.52
Hydrophobicity
- 0.142
Aliphatic Index
- 154.58
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 5
DRAMP03464
Comments Information
Function
- Has very low hemolytic activity on rat erythrocytes.
Literature Information
- ·Literature 1
-
Title
- Isolation and functional analysis of a 24-residue linear alpha-helical antimicrobial peptide from Korean blackish cicada, Cryptotympana dubia (Homoptera).
-
Pubmed ID
- 18000874
-
Reference
- Arch Insect Biochem Physiol. 2007 Dec;66(4):204-213.
-
Author
- Leem JY, Park DS, Suh EY, Hur JH, Oh HW, Park HY.