General Information

    • DRAMP ID

    • DRAMP03467

    • Peptide Name

    • Antibacterial napin (Plants)

    • Source

    • Brassica rapa subsp. chinensis (Pak-choi)

    • Family

    • Belongs to the 2S seed storage albumins family

    • Gene

    • Not found

    • Sequence

    • PAQPFRFPKHPQGPQTRPPI

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacterium: Pseudomonas fluorescens (MIC=66±3.7 µM);
      • Gram-positive bacteria: Bacillus subtilis (MIC=236±10.2 µM), Bacillus cereus (MIC=222±9.1 µM), Bacillus megaterium (MIC=215±6.8 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03467 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03467.

Physicochemical Information

    • Formula

    • C107H162N32O25

    • Absent Amino Acids

    • CDELMNSVWY

    • Common Amino Acids

    • P

    • Mass

    • 2296.66

    • PI

    • 12.01

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +4

    • Boman Index

    • -45.61

    • Hydrophobicity

    • -1.35

    • Aliphatic Index

    • 24.5

    • Half Life

      • Mammalian:>20 hour
      • Yeast:>20 hour
      • E.coli:?

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP03467

DRAMP03467 chydropathy plot

Comments Information

    • Function

    • The small, basic, water-soluble napins are one of the two major kinds of storage proteins synthesized in the seed during its maturation. Inhibits cell-free protein synthesis. Has antibacterial activity against Gram-negative bacterium P. fluorescens, and the Gram-positive bacteria B. subtilis, B. cereus, B. megaterium and M. phei. No antibacterial activity against the Gram-positive bacterium S. aureus or the Gram-negative bacteria E. coli, E. aerogenes and P. aeruginosa.

    • Subunit structure

    • The mature protein consists of a small and a large chain linked by disulfide bonds.

Literature Information

  • ·Literature 1

    • Title

    • A napin-like polypeptide from dwarf Chinese white cabbage seeds with translation-inhibitory, trypsin-inhibitory, and antibacterial activities.

    • Reference

    • Peptides. 2004 Feb;25(2):171-176.

    • Author

    • Ngai PH, Ng TB.