General Information

    • DRAMP ID

    • DRAMP03473

    • Peptide Name

    • Antimicrobial ribonuclease (Fungus)

    • Source

    • Pleurotus sajor-caju (Oyster mushroom)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • DNGEAGRAAR

    • Sequence Length

    • 10

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Antifungal

    • Target Organism

      • Bacteria: Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus aureus.
      • Fungi: Fusarium oxysporum (IC50=95 µM), Mycosphaerella arachidicola (IC50=72 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03473 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03473.

Physicochemical Information

    • Formula

    • C38H65N17O16

    • Absent Amino Acids

    • CFHIKLMPQSTVWY

    • Common Amino Acids

    • A

    • Mass

    • 1016.04

    • PI

    • 6.07

    • Basic Residues

    • 2

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 3

    • Net Charge

    • 0

    • Boman Index

    • -44.7

    • Hydrophobicity

    • -1.49

    • Aliphatic Index

    • 30

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP03473

Comments Information

    • Function

    • Has ribonuclease activity towards yeast tRNA and polyuracil and weak ribonuclease activity towards other polyhomoribonucleotides. Has weak deoxyribonuclease activity. Displays antimitogenic activity towards murine splenocytes and antiproliferative activity towards HepG2 hepatoma cells and L1210 leukemia cells.

    • Biophysicochemical properties

    • pH dependence (Optimum pH is from 5.5 to 6.0); Temperature dependence (Optimum temperature below 60 degrees Celsius. Active from 0 to 100 degrees Celsius).

Literature Information

  • ·Literature 1

    • Title

    • A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju.

    • Reference

    • Peptides. 2004 Jan;25(1):11-17.

    • Author

    • Ngai PH, Ng TB.