General Information

    • DRAMP ID

    • DRAMP03592

    • Peptide Name

    • Neutrophil defensin 2 (HNP-2, HP-2, HP2; Human, mammals, animals)

    • Source

    • Homo sapiens (Human)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • DEFA1 AND DEFA1B

    • Sequence

    • CYCRIPACIAGERRYGTCIYQGRLWAFCC

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.34206990]Virus:SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(approximately 50% inbibition at 1 μg/mL (290 nM)).
      • Gram-negative bacteria: Escherichia coli ATCC 8739 (vLD50=3.5±0.79 µg/ml), E. coli ATCC 25922 (vLD50=3.5±0.6 µg/ml), Enterobacter aerogenes ATCC 13048 (vLD50=16±4.0 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (vLD50=4.2±0.9 µg/ml), S. aureus ATCC 29213 (vLD50=2.4±0.3 µg/ml), Bacillus cereus ATCC 10876 (vLD50=0.22±0.04 µg/ml).(Ref.2)
      • NOTE: vLD50, virtual lethal doses (vLDs), equivalent to conventional 50% lethal doses (LD50s).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys1 and Cys29,Cys3 and Cys18,Cys8 and Cys28.

    • Stereochemistry

    • L

    • Structure

    • Beta strand

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03592 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP03592.

Physicochemical Information

    • Formula

    • C147H223N43O37S6

    • Absent Amino Acids

    • DHKMNSV

    • Common Amino Acids

    • C

    • Mass

    • 3377.01

    • PI

    • 8.67

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +3

    • Boman Index

    • -34.1

    • Hydrophobicity

    • 0.248

    • Aliphatic Index

    • 64.14

    • Half Life

      • Mammalian:1.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 10345

    • Absorbance 280nm

    • 369.46

    • Polar Residues

    • 13

DRAMP03592

DRAMP03592 chydropathy plot

Comments Information

    • Function

    • Defensin 2 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

    • PTM

    • Contains three disulfide bonds 1-29; 3-18; 8-28.

Literature Information

  • ·Literature 1

    • Title

    • Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

    • Reference

    • Viruses. 2021 Jun 26;13(7):1246.

    • Author

    • Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.
  • ·Literature 2

    • Title

    • Antibacterial activity and specificity of the six human {alpha}-defensins.

    • Reference

    • Antimicrob Agents Chemother. 2005 Jan;49(1):269-275.

    • Author

    • Ericksen B, Wu Z, Lu W, Lehrer RI.
  • ·Literature 3

    • Title

    • Reconstruction of the conserved beta-bulge in mammalian defensins using D-amino acids.

    • Reference

    • J Biol Chem. 2005 Sep 23;280(38):32921-9.

    • Author

    • Xie C, Prahl A, Ericksen B, Wu Z, Zeng P, Li X, Lu WY, Lubkowski J, Lu W.