General Information

    • DRAMP ID

    • DRAMP03603

    • Peptide Name

    • Human beta-defensin 28 (hBD-28; hBD28; Human, mammals, animals)

    • Source

    • Homo sapiens (Human)

    • Family

    • Belongs to the beta-defensin family

    • Gene

    • Not found

    • Sequence

    • ARLKKCFNKVTGYCRKKCKVGERYEIGCLSGKLCCAN

    • Sequence Length

    • 37

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli DSM1103 (MIC=37.5 µg/ml), Klebsiella pneumoniae DSM681 (MIC=50 µg/ml), Pseudomonas aeruginosa DSM1128 (MIC=37.5 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=25 µg/ml), Streptococcus pneumoniae DSM11865 (MIC=37.5 µg/ml).

    • Hemolytic Activity

      • [Ref.15625724] It is slightly hemolytic (<12%) against human erythrocytes at the highest concentration of 500 μg/ml.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys6 and Cys34,Cys14 and Cys28,Cys18 and Cys35

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03603 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C178H301N55O48S6

    • Absent Amino Acids

    • DHMPQW

    • Common Amino Acids

    • K

    • Mass

    • 4172.05

    • PI

    • 9.54

    • Basic Residues

    • 10

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +8

    • Boman Index

    • -70.96

    • Hydrophobicity

    • -0.4

    • Aliphatic Index

    • 63.24

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 93.19

    • Polar Residues

    • 16

DRAMP03603

DRAMP03603 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity gaginst Gram-positive and the Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Engineering disulfide bonds of the novel human beta-defensins hBD-27 and hBD-28: differences in disulfide formation and biological activity among human beta-defensins.

    • Reference

    • Biopolymers. 2005;80(1):34-49.

    • Author

    • Schulz A, Klüver E, Schulz-Maronde S, Adermann K.