General Information

    • DRAMP ID

    • DRAMP03722

    • Peptide Name

    • Cytotoxic linear peptide IsCT2

    • Source

    • Opisthacanthus madagascariensis (Scorpion)

    • Family

    • Belongs to the scorpion NDBP 5 family

    • Gene

    • Not found

    • Sequence

    • IFGAIWNGIKSLF

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.12054688] Gram-negetive bacteria: P.aeroginosa ATCC 15442 (MIC>150 μM), P.aeroginosa ATCC 27853 (MIC=68.3 μM), E.coli CCT 1371 (MIC=3.4 μM), Escherichia coli ATCC 25922 (MIC=6.8 μM), P. mirabilis (MIC>150 μM);
      • Gram-positive bacteria: S.aureus ATCC 25293 (MIC=0.7 μM), S.aureus ATCC 6538 (MIC=3.4 μM), S.saprophyticus (MIC=0.7 μM), B.thuringensis (MIC=17.1 μM), B.subtilis CCT 2471 (MIC=6.8μM).
      • [Ref.28657596] Gram-positive bacterium:Staphylococcus aureus (MIC=50μg/mL), B. cereus (MIC=100μg/mL);
      • Gram-negative bacterium: S. typhimurium (MIC=100μg/mL), Escherichia coli (MIC=50μg/mL)

    • Hemolytic Activity

      • [Ref.12054688] Showed relatively weak hemolytic activity against sheep red blood cells.
      • [Ref.28657596] 18% hemolysis at 10 μg/ml, 96% hemolysis at 50 μg/ml against human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03722 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C73H108N16O16

    • Absent Amino Acids

    • CDEHMPQRTVY

    • Common Amino Acids

    • I

    • Mass

    • 1465.76

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +1

    • Boman Index

    • 16.07

    • Hydrophobicity

    • 1.139

    • Aliphatic Index

    • 127.69

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 4

DRAMP03722

Comments Information

    • Function

    • IsCT2 showed broad activity spectra against microbes (Gram positive and negative bacteria as well as fungi). Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Purification, structure-Function: analysis, and molecular characterization of novel linear peptides from scorpion Opisthacanthus madagascariensis.

    • Reference

    • Biochem Biophys Res Commun. 2002 May 24;293(5):1514-1522.

    • Author

    • Dai L, Corzo G, Naoki H, Andriantsiferana M, Nakajima T.
  • ·Literature 2

    • Title

    • Antibacterial Activity and Toxicity of Analogs of Scorpion Venom IsCT Peptides

    • Reference

    • Antibiotics (Basel). 2017 Jun 28;6(3). pii: E13.

    • Author

    • de la Salud Bea R, Petraglia AF, Ascuitto MR, Buck QM.