• • DRAMP ID

  • DRAMP03751

• • Peptide Name

  • Venom antimicrobial peptide-9 (Meucin-18; NDBP-5; Arthropods, animals)

• • Source

  • Mesobuthus eupeus (Lesser Asian scorpion)

• • Family

  • Belongs to the ponericin-W family

• • Gene

  • Not found

• • Sequence

  • FFGHLFKLATKIIPSLFQ

• • Sequence Length

  • 18

• • UniProt Entry

  • E4VP50

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.19088182]Gram-positive bacteria: Bacillus sp. DM-1 (MIC=0.60 µM), Micrococcus luteus (MIC=0.60 µM), Bacillus megaterium (MIC=0.25 µM);
    • Gram-negative bacteria: Agrobacterium tumefaciens (MIC=6.00 µM), Escherichia coli (MIC=2.40 µM), Shewanella oneidensis (MIC=1.50 µM), Stenotrophomonus sp. YC-1(MIC=2.60 µM), Salmonella typhimurium (MIC=10.9 µM);
    • Fungi: Beauveria spp.(MIC=1.9 µM), Neurospora crassa (MIC=5.2 µM), Candida albicans (MIC=25.1 µM), Saccharomyces cerevisiae (MIC=25.1 µM), Aspergillus fumigatus (MIC=8.3 µM), Geotrichum candidum (MIC=3.5 µM).

• • Hemolytic Activity

  • • [Ref.19088182]74% hemolytic activity at 6.25 M, 100% hemolytic activity at 12.5 M against rabbit red blood cells

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP03751_predicted_structure.pdb to download.

DRAMP03751

• • Function

  • Amphipathic peptide that exhibits extensive cytolytic activities against both prokaryotic and eukaryotic cells. Is more potent against Gram-positive bacteria (lethal concentration (LC=1.5-10.9 µM) than against Gram-negative bacteria (LC=6.2-50 µM), and fungi (LC=25.1-8.3 µM). Is lethal to the fungus Beauveria sp (LC=1.9 µM), a highly lethal pathogenic fungus to insects and resistant to many AMPs. Shows hemolytic activity against rabbit erythrocytes and cytolysis against rat dorsal root ganglions. In vivo, intravenous injection into mice tail provokes uncomfortable symptoms with a death rate of 12.5%.

• • Tissue specificity

  • Expressed by the venom gland.

• ·Literature 1

• • Title

  • Structural and functional characterization of two genetically related meucin peptides highlights evolutionary divergence and convergence in antimicrobial peptides.

• • Pubmed ID

  • 19088182

• • Reference

  • FASEB J. 2009 Apr;23(4):1230-1245.

• • Author

  • Gao B, Sherman P, Luo L, Bowie J, Zhu S.