General Information
-
DRAMP ID
- DRAMP03779
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Peptide Name
- Antifungal protein (AFP; Fungi)
-
Source
- Aspergillus giganteus
-
Family
- Not found
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Gene
- afp
-
Sequence
- XTYNGKCYKKDNICKYKAQSGKTAICKCYVKKCPRDGAKCEFDSYKGKCYC
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Sequence Length
- 51
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UniProt Entry
- P17737
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antifungal
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Target Organism
- No MICs found in DRAMP database
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Phospholipids
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Beta strand (6 strands; 15 residues)
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Structure Description
- The folding of AFP consists of five antiparallel beta strands connected in a -1, -1, +3, +1 topology and highly twisted, defining a small and compact beta barrel stabilized by four internal disulfide bridges. A cationic site formed by up to three lysine side chains adjacent to a hydrophobic stretch, both at the protein surface, may constitute a potential binding site for phospholipids which would be the basis of its biological function.
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Helical Wheel Diagram
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PDB ID
- 1AFP resolved by NMR.
- 1AFP-> 
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Predicted Structure
- There is no predicted structure for DRAMP03779.
Physicochemical Information
-
Formula
- C248H390N68O71S8
Absent Amino Acids
- HLMW
Common Amino Acids
- K
Mass
- 5846.06
PI
- 9.27
Basic Residues
- 13
Acidic Residues
- 4
Hydrophobic Residues
- 7
Net Charge
- +9
-
Boman Index
- -109.8
Hydrophobicity
- -0.949
Aliphatic Index
- 26.86
Half Life
-
- Mammalian:?
- Yeast:?
- E.coli:?
Extinction Coefficient Cystines
- 9440
Absorbance 280nm
- 188.8
Polar Residues
- 24
DRAMP03779
Comments Information
Function
- This protein inhibits the growth of a variety of fungal species.
PTM
- Contains four disulfide bonds 7-33; 14-40; 26-28; 49-51.
Literature Information
- ·Literature 1
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Title
- Amino acid sequence and disulfide bridges of an antifungal protein isolated from Aspergillus giganteus.
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Pubmed ID
- 2226447
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Reference
- Eur J Biochem. 1990 Oct 5;193(1):31-38.
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Author
- Nakaya K, Omata K, Okahashi I, Nakamura Y, Kolekenbrock H, Ulbrich N.
- ·Literature 2
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Title
- NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism.
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Pubmed ID
- 7893713
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Reference
- Biochemistry. 1995 Mar 7;34(9):3009-3021.
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Author
- Campos-Olivas R, Bruix M, Santoro J, Lacadena J, Martinez del Pozo A, Gavilanes JG, Rico M.