• DRAMP ID

    • DRAMP03779
    • Peptide Name

    • Antifungal protein (AFP; Fungi)
    • Source

    • Aspergillus giganteus
    • Family

    • Unknown
    • Gene

    • afp
    • Sequence

    • XTYNGKCYKKDNICKYKAQSGKTAICKCYVKKCPRDGAKCEFDSYKGKCYC
    • Sequence Length

    • 51
    • Evidence code

    • Protein level
    • Biological Activity

    • Antifungal, Antimicrobial
    • Target Organism

      • No MICs found on DRAMP database
    • Hemolytic activity

    • Unknown
    • Binding Target

    • Phospholipids
    • Structure

    • Beta strand (6 strands; 15 residues)
    • Structure Description

    • The folding of AFP consists of five antiparallel beta strands connected in a -1, -1, +3, +1 topology and highly twisted, defining a small and compact beta barrel stabilized by four internal disulfide bridges. A cationic site formed by up to three lysine side chains adjacent to a hydrophobic stretch, both at the protein surface, may constitute a potential binding site for phospholipids which would be the basis of its biological function.
    • PDB ID

    • 1AFP resolved by NMR.
    • DRAMP03779 helical wheel diagram
    • 1AFP-> 
    • Formula

    • C248H390N68O71S8
    • Absent Amino Acids

    • HLMW
    • Common Amino Acids

    • K
    • Mass

    • 6718.76
    • PI

    • 9.31
    • Basic Residues

    • 13
    • Acidic Residues

    • 4
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -109.8
    • Hydrophobicity

    • -94.9
    • Aliphatic Index

    • 26.86
    • Half Life

      • Mammalian:?
      • Yeast:?
      • E.coli:?
    • Extinction Coefficient Cystines

    • 9440
    • Absorbance 280nm

    • 188.8
    • Polar Residues

    • 24

DRAMP03779

DRAMP03779 chydropathy plot
    • Function

    • This protein inhibits the growth of a variety of fungal species.
    • PTM

    • Contains four disulfide bonds 7-33; 14-40; 26-28; 49-51.
  • Literature 1
    • Reference

    • Eur J Biochem. 1990 Oct 5;193(1):31-38.
    • Author

    • Nakaya K, Omata K, Okahashi I, Nakamura Y, Kolekenbrock H, Ulbrich N.
    • Title

    • Amino acid sequence and disulfide bridges of an antifungal protein isolated from Aspergillus giganteus.
  • Literature 2
    • Reference

    • Biochemistry. 1995 Mar 7;34(9):3009-3021.
    • Author

    • Campos-Olivas R, Bruix M, Santoro J, Lacadena J, Martinez del Pozo A, Gavilanes JG, Rico M.
    • Title

    • NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism.