General Information

    • DRAMP ID

    • DRAMP03815

    • Peptide Name

    • D16W-N23 (single amino acid substitution)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GILDTLKQFAKGVGKWLVKGAAQ

    • Sequence Length

    • 23

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Micrococcus luteus (MIC=2.5 µg/ml), Bacillus subtilis (MIC=25 µg/ml);
      • Gram-negative bacteria: Klebsiella pneumoniae (MIC=25 µg/ml), Shigella dysentariae (MIC=50 µg/ml), Pseudomonas aeruginosa (MIC=125 µg/ml), Eshchericia coli (MIC=25 µg/ml), Salmonella typhimurium (MIC=125 µg/ml).

    • Hemolytic Activity

      • [Ref.12199716] 0.02% hemolytic activity at 10 µg/mL, 0.38% hemolytic activity at 100 µg/mL against human red blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03815 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03815.

Physicochemical Information

    • Formula

    • C113H186N30O29

    • Absent Amino Acids

    • CEHMNPRSY

    • Common Amino Acids

    • GK

    • Mass

    • 2428.9

    • PI

    • 10

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • -2.31

    • Hydrophobicity

    • 0.139

    • Aliphatic Index

    • 106.09

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 250

    • Polar Residues

    • 5

DRAMP03815

DRAMP03815 chydropathy plot

Comments Information

    • Comment

    • Single substitution of aspartic acid 16 by tryptophan (D16W) in the Delta24-37 GGN4, which is a peptide analogue with 14 residues truncated from the C-terminus of GGN4.

Literature Information

  • ·Literature 1

    • Title

    • Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4.

    • Reference

    • Eur J Biochem. 2002 Sep;269(17):4367-4374.

    • Author

    • Won HS, Park SH, Kim HE, Hyun B, Kim M, Lee BJ, Lee BJ.