General Information

    • DRAMP ID

    • DRAMP03863

    • Peptide Name

    • Sub3 (Bac2A variant through three amino acids substitution)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RRWRIVVIRVRR

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Pseudomonas aeruginosa (MIC=2 µg/ml), Escherichia coli (MIC=2.5 µg/ml), Salmonella typhimurium (MIC=4 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=2 µg/ml), Staphylococcus epidermidis (MIC=0.2 µg/ml), Enterococcus faecalis (MIC=4 µg/ml).
      • Yeast: Candida albicans (MIC=4 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03863 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C74H133N31O13

    • Absent Amino Acids

    • ACDEFGHKLMNPQSTY

    • Common Amino Acids

    • R

    • Mass

    • 1665.07

    • PI

    • 12.7

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +6

    • Boman Index

    • -65.23

    • Hydrophobicity

    • -0.525

    • Aliphatic Index

    • 137.5

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 500

    • Polar Residues

    • 0

DRAMP03863

Comments Information

    • Function

    • Optimized 12-mer peptides shows broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.

    • Half Life

    • With a half-life of approximately 1h in 25% aqueous mouse serum,Sub3 was relatively stable for a medium-sized peptide.

    • Chemical modification

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • High-throughput generation of small antibacterial peptides with improved activity.

    • Reference

    • Nat Biotechnol. 2005 Aug;23(8):1008-1012.

    • Author

    • Hilpert K, Volkmer-Engert R, Walter T, Hancock RE.