General Information

    • DRAMP ID

    • DRAMP03927

    • Peptide Name

    • Cecropin A (1-7)-melittin (2-9)hybrid peptide

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KWKLFKKIGAVLKVL

    • Sequence Length

    • 15

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli D21 (MIC=1 µM), Pseudomonas aeruginosa OT97 (MIC=4 µM);
      • Gram-positive bacteria: Bacillus subtilis Bs11 (MIC=0.5 µM), Bacillus megaterium Bm11 (MIC=0.7 µM), Staphylococcus aureus Cowan 1 (MIC=2 µM), Streptococcus pyogenes (MIC=0.5 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (1 helices; 10 residues)

    • Structure Description

    • Adopts an amphipathic helix almost parallel to the surface of the micelle.

    • Helical Wheel Diagram

    • DRAMP03927 helical wheel diagram

    • PDB ID

    • 2JMY resolved by NMR.
  • 2JMY-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C89H151N21O16

    • Absent Amino Acids

    • CDEHMNPQRSTY

    • Common Amino Acids

    • K

    • Mass

    • 1771.31

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +5

    • Boman Index

    • 8.07

    • Hydrophobicity

    • 0.54

    • Aliphatic Index

    • 149.33

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 392.86

    • Polar Residues

    • 1

DRAMP03927

Comments Information

    • CAMEL is also a hybrid peptide obtained by combining residues 1-7 of cecropin (CA) and residues 2-9 of melittin (M).

Literature Information

  • ·Literature 1

    • Title

    • Shortened cecropin A-melittin hybrids. Significant size reduction retains potent antibiotic activity.

    • Reference

    • FEBS Lett. 1992 Jan 20;296(2):190-194.

    • Author

    • Andreu D, Ubach J, Boman A, WÃ¥hlin B, Wade D, Merrifield RB, Boman HG.
  • ·Literature 2

    • Title

    • Mapping the orientation of helices in micelle-bound peptides by paramagnetic relaxation waves.

    • Reference

    • J Am Chem Soc. 2007 Apr 25;129(16):5228-5234.

    • Author

    • Respondek M, Madl T, Göbl C, Golser R, Zangger K.