General Information
Activity Information
-
Biological Activity
- Antimicrobial, Antifungal
-
Target Organism
-
- SMF- (synthetic low ionic strength medium): Fusarium culmorum (IC50=4.0±0.2);
- SMF+ (SMF + 1 mM CaCl2 and 50 mM KCl): Fusarium culmorum (IC50=3.2±0.3).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP03953.
Physicochemical Information
-
Formula
- C245H382N80O68S8
Absent Amino Acids
- DM
Common Amino Acids
- C
Mass
- 5792.7
PI
- 9.25
Basic Residues
- 10
Acidic Residues
- 1
Hydrophobic Residues
- 11
Net Charge
- +9
-
Boman Index
- -109.44
Hydrophobicity
- -0.641
Aliphatic Index
- 42.16
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8980
Absorbance 280nm
- 179.6
Polar Residues
- 23
DRAMP03953
Comments Information
Compared to Rs-AFP2, the mutation Rs-AFP2(V39R) has increased antifungal potency.
Literature Information
- ·Literature 1
-
Title
- Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity.
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Pubmed ID
- 8995418
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Reference
- J Biol Chem. 1997 Jan 10;272(2):1171-1179.
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Author
- De Samblanx GW, Goderis IJ, Thevissen K, Raemaekers R, Fant F, Borremans F, Acland DP, Osborn RW, Patel S, Broekaert WF.