• DRAMP ID

    • DRAMP03953
    • Peptide Name

    • Rs-AFP2 variant (Mutation: V39R)
    • Source

    • Synthetic construct (Mutation of Rs-AFP2)
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • QKLCQRPSGTWSGVCGNNNACKNQCIRLEKARHGSCNYRFPAHKCICYFPC
    • Sequence Length

    • 51
    • Protein Existence

    • Synthetic
    • Biological Activity

    • Antimicrobial, Antifungal
    • Target Organism

      • SMF- (synthetic low ionic strength medium): Fusarium culmorum (IC50=4.0±0.2);
      • SMF+ (SMF + 1 mM CaCl2 and 50 mM KCl): Fusarium culmorum (IC50=3.2±0.3).
    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP03953 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP03953.
    • Formula

    • C245H382N80O68S8
    • Absent Amino Acids

    • DM
    • Common Amino Acids

    • C
    • Mass

    • 5792.7
    • PI

    • 9.25
    • Basic Residues

    • 10
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 11
    • Net Charge

    • +9
    • Boman Index

    • -109.44
    • Hydrophobicity

    • -0.641
    • Aliphatic Index

    • 42.16
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 8980
    • Absorbance 280nm

    • 179.6
    • Polar Residues

    • 23

DRAMP03953

DRAMP03953 chydropathy plot
    • Compared to Rs-AFP2, the mutation Rs-AFP2(V39R) has increased antifungal potency.

  • ·Literature 1
    • Title

    • Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity.
    • Reference

    • J Biol Chem. 1997 Jan 10;272(2):1171-1179.
    • Author

    • De Samblanx GW, Goderis IJ, Thevissen K, Raemaekers R, Fant F, Borremans F, Acland DP, Osborn RW, Patel S, Broekaert WF.