General Information

    • DRAMP ID

    • DRAMP03998

    • Peptide Name

    • PAF26 (Trp-rich; combinatorial library)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RKKWFW

    • Sequence Length

    • 6

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.11976121] Gram-negative bacterium: Escherichia coli DH5α (MIC=160 µM, IC50>80 µM);
      • Fungi: Penicillium digitatum PHI-26 (MIC=20 µM, IC50=11 ± 1 µM), Penicillium italicum PHI-1 (MIC=40 µM, IC50=22 ± 3 µM), Botrytis cinerea CECT2100 (MIC=60 µM, IC50=38 ± 24 µM), Fusarium oxysporum CECT2866 (MIC=80 µM, IC50=45 ± 4 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Rich

    • Structure Description

    • In general, positively charged residues (arginine or lysine) and residues with aromatic rings (tryptophan or phenylalanine) rendered antifungal activity.

    • Helical Wheel Diagram

    • DRAMP03998 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03998.

Physicochemical Information

    • Formula

    • C49H67N13O7

    • Absent Amino Acids

    • ACDEGHILMNPQSTVY

    • Common Amino Acids

    • KW

    • Mass

    • 950.15

    • PI

    • 11.17

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +3

    • Boman Index

    • -18.38

    • Hydrophobicity

    • -1.883

    • Aliphatic Index

    • 0

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 2200

    • Polar Residues

    • 0

DRAMP03998

Comments Information

    • Function

    • Antibacterial against Gram-negative bacterium. The antifungal mixture is also antibacterial, with a predominance of basic and aromatic residues.

Literature Information

  • ·Literature 1

    • Title

    • Identification of novel hexapeptides bioactive against phytopathogenic fungi through screening of a synthetic peptide combinatorial library.

    • Reference

    • Appl Environ Microbiol. 2002 May;68(5):2453-60.

    • Author

    • López-García B, Pérez-Payá E, Marcos JF.