General Information

    • DRAMP ID

    • DRAMP03999

    • Peptide Name

    • [A6]-IsCT (Mutant: W6A; IsCT analog)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILGKIAEGIKSLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.15369808]Gram-negative bacteria: Escherichia coli KCTC 1682 (MIC=64 µM), Pseudomonas aeruginosa KCTC 1637 (MIC=32 µM), Salmonella typhimurium KCTC 1926 (MIC>64 µM);
      • Gram-positive bacteria: Staphylococcus epidermidis KCTC 1917 (MIC>64 µM), Staphylococcus aureus KCTC 1621 (MIC>64 µM).

    • Hemolytic Activity

      • [Ref.15369808]Non-hemolytic activity

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03999 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03999.

Physicochemical Information

    • Formula

    • C66H113N15O17

    • Absent Amino Acids

    • CDHMNPQRTVWY

    • Common Amino Acids

    • I

    • Mass

    • 1388.71

    • PI

    • 8.59

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +1

    • Boman Index

    • 9.96

    • Hydrophobicity

    • 0.985

    • Aliphatic Index

    • 157.69

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP03999

Comments Information

    • Function

    • [A6]-IsCT did not cause any hemolysis at peptide concentrations as high as 100 µM, indicating that Trp6 is important for both the hemolytic activity and antibacterial activity of IsCT.

    • Chemical modification

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs.

    • Reference

    • Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-719.

    • Author

    • Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y.