General Information

    • DRAMP ID

    • DRAMP04000

    • Peptide Name

    • [L6]-IsCT (Mutant: W6L; IsCT analog)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILGKILEGIKSLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Escherichia coli KCTC 1682 (MIC=16 µM), Pseudomonas aeruginosa KCTC 1637 (MIC=16 µM), Salmonella typhimurium KCTC 1926 (MIC=16 µM);
      • Gram-positive bacteria: Staphylococcus epidermidis KCTC 1917 (MIC=32 µM), Staphylococcus aureus KCTC 1621 (MIC=4 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP04000 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C69H119N15O17

    • Absent Amino Acids

    • ACDHMNPQRTVWY

    • Common Amino Acids

    • IL

    • Mass

    • 1430.79

    • PI

    • 8.59

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +1

    • Boman Index

    • 13.07

    • Hydrophobicity

    • 1.139

    • Aliphatic Index

    • 180

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP04000

Comments Information

    • Function

    • Drastic reduction in antibacterial activity against both Gram-positive and Gram-negative bacteria, and big loss of hemolysis activity.

    • Chemical modification

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs.

    • Reference

    • Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-719.

    • Author

    • Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y.