General Information

    • DRAMP ID

    • DRAMP04003

    • Peptide Name

    • [K7, P8, K11]-IsCT (IsCT analog through amino acids substitution)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILGKIWKIKKLF

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.15369808]Gram-negative bacteria: Escherichia coli KCTC 1682 (MIC=2 µM), Pseudomonas aeruginosa KCTC 1637 (MIC=2 µM), Salmonella typhimurium KCTC 1926 (MIC=1 µM);
      • Gram-positive bacteria: Staphylococcus epidermidis KCTC 1917 (MIC=1 µM), Staphylococcus aureus KCTC 1621 (MIC=2 µM);
      • Antibiotic-resistant bacteria: Methicillin-resistant Staphylococcus aureus (MRSA) CCARM 3001 (MIC=1 µM), multidrug-resistant Pseudomonas aeruginosa (MDRPA) (CCARM 3543) (MIC=1 µM) and multidrug-resistant Pseudomonas aeruginosa (MDRPA) CCARM 2095 (MIC=1 µM).

    • Hemolytic Activity

      • [Ref.15369808]Non-hemolytic activity

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP04003 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C76H127N17O13

    • Absent Amino Acids

    • ACDEHMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1486.95

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • 8.65

    • Hydrophobicity

    • 0.583

    • Aliphatic Index

    • 162.5

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 500

    • Polar Residues

    • 1

DRAMP04003

Comments Information

    • Function

    • [K7, P8, K11]-IsCT exhibited more potent antibacterial activity than native IsCT but no hemolytic activity, even at concentrations as high as 100 µM. [K7, P8, K11]-IsCT displays a similar or 2-fold higher antibacterial activity relative to that of native IsCT.

    • Chemical modification

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Antibiotic activity and structural analysis of the scorpion-derived antimicrobial peptide IsCT and its analogs.

    • Reference

    • Biochem Biophys Res Commun. 2004 Oct 15;323(2):712-719.

    • Author

    • Lee K, Shin SY, Kim K, Lim SS, Hahm KS, Kim Y.