General Information

    • DRAMP ID

    • DRAMP04076

    • Peptide Name

    • Anal 1 (antimicrobial peptide HP (2-20)analogue)

    • Source

    • Synthetic construct (amino acid substitution)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • AKKVFKRLEKLFSKIQNWK

    • Sequence Length

    • 19

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria:
        Target OrganismActivity
        Bacillus subtilisMIC=1.56 µg/ml
        Staphylococcus epidermidisMIC=3.13 µg/ml
        Staphylococcus aureusMIC=3.13 µg/ml
        Listeria monocytogenes (MIC=0.78 µg/ml);-
      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coliMIC=0.78 µg/ml
        Salmonella typhimuriumMIC=0.39 µg/ml
        Proteus vulgarisMIC=3.13 µg/ml
        Pseudomonas aeruginosaMIC=3.13 µg/ml
        Escherichia coli O157MIC=6.25 µg/ml
      • Fungi: Trichosporon beigelii (MIC=1.56 µg/ml), Candida albicans (MIC=25 µg/ml), Saccharomyces cerevisiae (MIC=25 µg/ml), Aspergillus flavus (MIC=50 µg/ml), Aspergillus fumigatus (MIC>50 µg/ml), Botrytis cinerea (MIC=6.25 µg/ml), Fusarium moniliforme (MIC=12.5 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix (1 helices; 14 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP04076 helical wheel diagram

    • PDB ID

    • 1P0J resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP04076.

Physicochemical Information

    • Formula

    • C114H187N31O25

    • Absent Amino Acids

    • CDGHMPTY

    • Common Amino Acids

    • K

    • Mass

    • 2391.93

    • PI

    • 10.68

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +6

    • Boman Index

    • -41.71

    • Hydrophobicity

    • -0.863

    • Aliphatic Index

    • 82.11

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 305.56

    • Polar Residues

    • 2

DRAMP04076

DRAMP04076 chydropathy plot

Comments Information

    • Function

    • Has antibacterial and antifungal activiy.

Literature Information

  • ·Literature 1

    • Title

    • Interactions between the plasma membrane and the antimicrobial peptide HP (2-20) and its analogues derived from Helicobacter pylori.

    • Reference

    • Biochem J. 2006 Feb 15;394(Pt 1):105-114.

    • Author

    • Lee KH, Lee DG, Park Y, Kang DI, Shin SY, Hahm KS, Kim Y.
  • ·Literature 2

    • Title

    • Interactions between antimicrobial peptide, HP(2-20) derived from helicobacter pylori, and membrain studied by nmr spectroscopy.

    • Reference

    • To be published

    • Author

    • Lee KH, Lee DG, Park YK, Harm KS, Kim YM.