General Information
-
DRAMP ID
- DRAMP04691
-
Peptide Name
- Parigidin-br1
-
Source
- Palicourea rigida
-
Family
- Belongs to the cyclotide family (Bracelet subfamily)
-
Gene
- Not found
-
Sequence
- GGSVPCGESCVFIPCITSLAGCSCKNKVCYYD
-
Sequence Length
- 32
-
UniProt Entry
- B3EWF1
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Toxin
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Target Organism
- [Swiss_Prot Entry B3EWF1]No antibacterial activity against E.coli strain ATCC 8739 and S.aureus strain ATCC 25923
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Hemolytic Activity
-
- [Ref.22074926] It has 28% and 41% hemolysis at 20 μM and 40 μM against human red blood cells.
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Cytotoxicity
-
- [Ref.22074926] It exhibited cytotoxic effects on SF-9 cells with CC50 values to be 1.7 μm at 24 h, 10.3 μm at 48 h and 73.1 μm at 72 h.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys6 and Cys22,Cys10 and Cys24,Cys15 and Cys29.
-
Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP04691.
Physicochemical Information
-
Formula
- C140H219N35O45S6
Absent Amino Acids
- HMQRW
Common Amino Acids
- C
Mass
- 3304.85
PI
- 6.03
Basic Residues
- 2
Acidic Residues
- 2
Hydrophobic Residues
- 8
Net Charge
- 0
-
Boman Index
- -661
Hydrophobicity
- 0.481
Aliphatic Index
- 66.88
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 3355
Absorbance 280nm
- 108.23
Polar Residues
- 18
DRAMP04691
Comments Information
Function
- Probably participates in a plant defense mechanism. Reduces growth of and increases mortality in larvae of D.saccharalis. Kills cultured SF-9 cells of S.frugiperda probably by disrupting plasma membranes. Has hemolytic activity against human erythrocytes. Has no antibacterial activity against E.coli strain ATCC 8739 and S.aureus strain ATCC 25923.
Tissue specificity
- Expressed in leaves, flowers, peduncles and seeds (at protein level).
PTM
- This is a cyclic peptide.
Literature Information
- ·Literature 1
-
Title
- Identification and structural characterization of novel cyclotide with activity against an insect pest of sugar cane.
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Pubmed ID
- 22074926
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Reference
- J Biol Chem. 2012 Jan 2;287(1):134-147.
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Author
- Pinto MF, Fensterseifer IC, Migliolo L, Sousa DA, de Capdville G, Arboleda-Valencia JW, Colgrave ML, Craik DJ, Magalhães BS, Dias SC, Franco OL.