• DRAMP ID

    • DRAMP04691
    • Peptide Name

    • Parigidin-br1
    • Source

    • Palicourea rigida
    • Family

    • Belongs to the cyclotide family (Bracelet subfamily)
    • Gene

    • Not found
    • Sequence

    • GGSVPCGESCVFIPCITSLAGCSCKNKVCYYD
    • Sequence Length

    • 32
    • Protein Existence

    • Protein level
    • Biological Activity

    • Toxin
    • Target Organism

    • [Swiss_Prot Entry B3EWF1]No antibacterial activity against E.coli strain ATCC 8739 and S.aureus strain ATCC 25923
    • Hemolytic Activity

      • [Ref.22074926] It has 28% and 41% hemolysis at 20 μM and 40 μM against human red blood cells.
    • Cytotoxicity

      • [Ref.22074926] It exhibited cytotoxic effects on SF-9 cells with CC50 values to be 1.7 μm at 24 h, 10.3 μm at 48 h and 73.1 μm at 72 h.
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • No specific N-terminal
    • C-terminal Modification

    • No specific C-terminal
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys6 and Cys22,Cys10 and Cys24,Cys15 and Cys29.
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP04691 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP04691.
    • Formula

    • C140H219N35O45S6
    • Absent Amino Acids

    • HMQRW
    • Common Amino Acids

    • C
    • Mass

    • 3304.85
    • PI

    • 6.03
    • Basic Residues

    • 2
    • Acidic Residues

    • 2
    • Hydrophobic Residues

    • 8
    • Net Charge

    • 0
    • Boman Index

    • -661
    • Hydrophobicity

    • 0.481
    • Aliphatic Index

    • 66.88
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 3355
    • Absorbance 280nm

    • 108.23
    • Polar Residues

    • 18

DRAMP04691

DRAMP04691 chydropathy plot
    • Function

    • Probably participates in a plant defense mechanism. Reduces growth of and increases mortality in larvae of D.saccharalis. Kills cultured SF-9 cells of S.frugiperda probably by disrupting plasma membranes. Has hemolytic activity against human erythrocytes. Has no antibacterial activity against E.coli strain ATCC 8739 and S.aureus strain ATCC 25923.
    • Tissue specificity

    • Expressed in leaves, flowers, peduncles and seeds (at protein level).
    • PTM

    • This is a cyclic peptide.
  • ·Literature 1
    • Title

    • Identification and structural characterization of novel cyclotide with activity against an insect pest of sugar cane.
    • Reference

    • J Biol Chem. 2012 Jan 2;287(1):134-147.
    • Author

    • Pinto MF, Fensterseifer IC, Migliolo L, Sousa DA, de Capdville G, Arboleda-Valencia JW, Colgrave ML, Craik DJ, Magalhães BS, Dias SC, Franco OL.