General Information
-
DRAMP ID
- DRAMP18195
-
Peptide Name
- LsbB (Bacteriocin)
-
Source
- Lactococcus lactis subsp. lactis (Streptococcus lactis)
-
Family
- Not found
-
Gene
- lsbB
-
Sequence
- MKTILRFVAGYDIASHKKKTGGYPWERGKA
-
Sequence Length
- 30
-
UniProt Entry
- Q7X2B5
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Protein Existence
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial
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Target Organism
- targeting primarily lactococcal cells
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
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Structure
- alpha helical
-
Structure Description
- Structure determination by NMR spectroscopy showed that LsbB has an N-terminal alpha helix, whereas the C-terminal of the molecule remains unstructured.
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP18195.
Physicochemical Information
-
Formula
- C156H246N44O40S
Absent Amino Acids
- CNQ
Common Amino Acids
- K
Mass
- 3410
PI
- 10.12
Basic Residues
- 8
Acidic Residues
- 2
Hydrophobic Residues
- 9
Net Charge
- +6
-
Boman Index
- -5095
Hydrophobicity
- -0.683
Aliphatic Index
- 58.67
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8480
Absorbance 280nm
- 292.41
Polar Residues
- 9
DRAMP18195
Comments Information
The results indicate that the outmost eight-amino acid sequence at the C-terminal end is likely to contain the receptor binding domain. Alanine substitution revealed that the tryptophan in position 25 (Trp25) is crucial for the blocking activity of the truncated peptides, as well as for the antimicrobial activity of the full-length bacteriocin.Â
Literature Information
- ·Literature 1
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Title
- Novel mechanism of bacteriocin secretion and immunity carried out bylactococcal multidrug resistance proteins.
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Pubmed ID
- 12801935
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Reference
- J. Biol. Chem. 278:34291-34298(2003).
-
Author
- Gajic O., Buist G., Kojic M., Topisirovic L., Kuipers O.P., Kok J.
- ·Literature 2
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Title
- Defining the structure and receptor binding domain of the leaderlessbacteriocin LsbB.
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Pubmed ID
- 24993828
-
Reference
- J. Biol. Chem. 289:23838-23845(2014)
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Author
- Ovchinnikov K.V., Kristiansen P.E., Uzelac G., Topisirovic L.,Kojic M., Nissen-Meyer J., Nes I.F., Diep D.B.