General Information

    • DRAMP ID

    • DRAMP18209

    • Peptide Name

    • NAI-802 (Bacteriocin)

    • Source

    • Actinoplanes strains ID104802 and ID104771

    • Family

    • Belongs to the lantibiotics family (Class I bacteriocin)

    • Gene

    • Not found

    • Sequence

    • ASSGWVCTLTIECGTVICACR

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

    • Gram-positive

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Lipid II

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • This peptide shares 19 residues with class II lantibiotic actagardine. In addition, it contains one extra Ala at the N-terminus and an extra Arg at the C-terminus. Actagardine, Ala(0)-actagardine, actagardine B, michiganin A, NAI-802 and Ala(0)-NAI-802 share three conserved thioether bridges within a core 19-aa peptide consisting of 14 invariant residues.

    • Helical Wheel Diagram

    • DRAMP18209 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18209.

Physicochemical Information

    • Formula

    • C90H149N25O29S4

    • Absent Amino Acids

    • DFHKMNPQY

    • Common Amino Acids

    • C

    • Mass

    • 2173.56

    • PI

    • 6.01

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • 0

    • Boman Index

    • -45

    • Hydrophobicity

    • 1.019

    • Aliphatic Index

    • 92.86

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5750

    • Absorbance 280nm

    • 287.5

    • Polar Residues

    • 11

DRAMP18209

DRAMP18209 chydropathy plot

Comments Information

    • As expected from the overall higher positive charge, NAI-802 was slightly more active than actagardine against staphylococci and streptococci. Further improvement of its antibacterial activity was achieved by adding one additional positive charge through conversion of the C-terminal carboxylate into the corresponding basic amide.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and characterization of NAI-802, a new lantibiotic produced by two different Actinoplanes strains.

    • Reference

    • J Antibiot (Tokyo). 2013 Feb;66(2):73-8.

    • Author

    • Simone M, Monciardini P, Gaspari E, Donadio S, Maffioli SI.