General Information

    • DRAMP ID

    • DRAMP18320

    • Peptide Name

    • Epilancin 15X(Bacteriocin)

    • Source

    • Staphylococcus epidermidis 15X154

    • Family

    • Belongs to the lantibiotics family (Class I bacteriocin)

    • Gene

    • Not found

    • Sequence

    • SASIVKTTIKASKKLCRGFTLTCGCHFTGKK

    • Sequence Length

    • 31

    • Protein Existence

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

    • Gram-positive

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Non helix or strand structure

    • Structure Description

    • The peptide was found to highly resemble the previously identified epilancin K7 with 68% sequence identity, three nearly identical lanthionine rings and a modified amino acid at the N-terminus. The C-terminal B and C rings of epilancin 15X are structurally similar to the D and E rings of nisin A that are believed to be involved in pore formation.

    • Helical Wheel Diagram

    • DRAMP18320 helical wheel diagram

    • PDB ID

    • 1W9N resolved by NMR

    • Predicted Structure

    • There is no predicted structure for DRAMP18320.

Physicochemical Information

    • Formula

    • C145H248N42O40S3

    • Absent Amino Acids

    • DEMNPQWY

    • Common Amino Acids

    • K

    • Mass

    • 3316

    • PI

    • 10.07

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +8

    • Boman Index

    • -3597

    • Hydrophobicity

    • -0.023

    • Aliphatic Index

    • 66.13

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.17

    • Polar Residues

    • 14

DRAMP18320

DRAMP18320 chydropathy plot

Comments Information

    • The compound contains an unusual N-terminal D-lactate group that could be essential for biological activity. This study demonstrates that this moiety confers stability against proteolytic degradation by aminopeptidases.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and structural characterization of epilancin 15X, a novel lantibiotic from a clinical strain of Staphylococcus epidermidis.

    • Reference

    • FEBS Lett. 2005 Mar 28;579(9):1917-22.

    • Author

    • Ekkelenkamp MB, Hanssen M, Danny Hsu ST, de Jong A, Milatovic D, Verhoef J, van Nuland NA.
  • ·Literature 2

    • Title

    • Biosynthesis of the antimicrobial peptide epilancin 15X and its N-terminal lactate.

    • Reference

    • Chem Biol. 2011 Jul 29;18(7):857-67.

    • Author

    • Vel