• • DRAMP ID

  • DRAMP18495

• • Peptide Name

  • Gomesin (Gm; Spiders, arachnids, Chelicerata, arthropods, invertebrates, animals)

• • Source

  • Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus)

• • Family

  • Belongs to the tachyplesin family

• • Gene

  • Not found

• • Sequence

  • QCRRLCYKQRCVTYCRGR

• • Sequence Length

  • 18

• • UniProt Entry

  • P82358

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiparasitic, Antimalarial, Antic

• • Target Organism

  • • [Ref.10942757] Gram-positive bacteria: Aerococcus viridans (MIC=0.8-1.6 μM), Bacillus cereus (MIC=6.25-12.5 μM), Bacillus megaterium (MIC=0.2-0.4 μM), Bacillus thuringiensis (MIC=1.6-3.15 μM), Enterococcus faecalis (MIC=6.2-12.5 μM), Listeria monocytogenes (MIC=0.8-1.6 μM), Micrococcus luteus (MIC=0.4-0.8 μM), Pediococcus acidolacrici (MIC=3.15-6.25 μM), Staphylococcus aureus (MIC=1.6-3.15 μM), Staphylococcus epidermidis (MIC=0.8-1.6 μM), Staphylococcus haemolyticus (MIC=0.8-1.6 μM), Staphylococcus saprophyticus (MIC=0.8-1.6 μM), Streptococcus pyogenes (MIC=1.6-3.15 μM), Nocardia asteroides (MIC=1.6-3.15 μM);
    • Gram-negative bacteria: Escherichia coli 1106 (MIC=0.8-1.6 μM), Escherichia coli D22 (MIC=0.4-0.8 μM), Escherichia coli D31 (MIC=0.8-1.6 μM), Escherichia coli SBS363 (MIC=0.4-0.8 μM), Erwinia carolovora (MIC=3.15-6.25 μM), Enterobacter cloacae β12 (MIC=3.15-6.25 μM), Klebsiella pneumoniae (MIC=3.15-6.25 μM), Pseudomonas aeruginosa (MIC=1.6-3.15 μM), Salmonella thyphinurium (MIC=0.8-1.6 μM), Xhantomonas campestris pv. orizae (MIC=3.15-6.25 μM);
    • Fungi: Alternaria brassicola (MIC=0.4-0.8 μM), Aspergillus fumigatus (MIC=1.6-3.15 μM), Beauveria bassiana (MIC=12.5-25 μM), Fusarium culmorum (MIC=0.4-0.8 μM), Fusarium oxysporum (MIC=0.4-0.8 μM), Neurospora crassa (MIC=0.4-0.8 μM), Nectria haematococca (MIC=0.2-0.4 μM), Tricoderma viridae (MIC=0.4-0.8 μM), Tricophvton mentagrophytes (MIC=0.8-1.6 μM);
    • Yeasts: Candida albicans (MIC=0.15-0.3 μM), Candida glabrata (MIC=12.5-25 μM), Candida tropicalis (MIC=3.15-6.25 μM), Cryptococcus neoformans (MIC=0.8-1.6 μM), Saccharomyces cerevisiae (MIC=1.6-3.15 μM).

• • Hemolytic Activity

  • • [Ref.10942757] It exhibits 16% hemolysis at 1 μM and 100 μM against human erythrocytes

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • pyroglutamic acid

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys2 and Cys15,Cys6 and Cys11.

• • Stereochemistry

  • L

• • Structure

  • Beta sheet

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1KFP resolved by NMR.
• 1KFP-> 

• Predicted Structure

• There is no predicted structure for DRAMP18495.

DRAMP18495

• • Function

  • Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. It shows hemolytic activity.

• • Tissue specificity

  • In hemocytes only, but not in all hemocytes observed.

• ·Literature 1

• • Title

  • Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family.

• • Pubmed ID

  • 10942757

• • Reference

  • J Biol Chem. 2000 Oct 27;275(43):33464-70.

• • Author

  • Silva PI Jr, Daffre S, Bulet P.