• DRAMP ID

    • DRAMP18495
    • Peptide Name

    • Gomesin (Gm; Spiders, arachnids, Chelicerata, arthropods, invertebrates, animals)
    • Source

    • Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus)
    • Family

    • Belongs to the tachyplesin family
    • Gene

    • Unknown
    • Sequence

    • QCRRLCYKQRCVTYCRGR
    • Sequence Length

    • 18
    • Evidence code

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiparasitic, Antimalarial, Antic
    • Target Organism

      • [Ref.10942757] Gram-positive bacteria: Aerococcus viridans (MIC=0.8-1.6 μM), Bacillus cereus (MIC=6.25-12.5 μM), Bacillus megaterium (MIC=0.2-0.4 μM), Bacillus thuringiensis (MIC=1.6-3.15 μM), Enterococcus faecalis (MIC=6.2-12.5 μM), Listeria monocytogenes (MIC=0.8-1.6 μM), Micrococcus luteus (MIC=0.4-0.8 μM), Pediococcus acidolacrici (MIC=3.15-6.25 μM), Staphylococcus aureus (MIC=1.6-3.15 μM), Staphylococcus epidermidis (MIC=0.8-1.6 μM), Staphylococcus haemolyticus (MIC=0.8-1.6 μM), Staphylococcus saprophyticus (MIC=0.8-1.6 μM), Streptococcus pyogenes (MIC=1.6-3.15 μM), Nocardia asteroides (MIC=1.6-3.15 μM);
      • Gram-negative bacteria: Escherichia coli 1106 (MIC=0.8-1.6 μM), Escherichia coli D22 (MIC=0.4-0.8 μM), Escherichia coli D31 (MIC=0.8-1.6 μM), Escherichia coli SBS363 (MIC=0.4-0.8 μM), Erwinia carolovora (MIC=3.15-6.25 μM), Enterobacter cloacae β12 (MIC=3.15-6.25 μM), Klebsiella pneumoniae (MIC=3.15-6.25 μM), Pseudomonas aeruginosa (MIC=1.6-3.15 μM), Salmonella thyphinurium (MIC=0.8-1.6 μM), Xhantomonas campestris pv. orizae (MIC=3.15-6.25 μM);
      • Fungi: Alternaria brassicola (MIC=0.4-0.8 μM), Aspergillus fumigatus (MIC=1.6-3.15 μM), Beauveria bassiana (MIC=12.5-25 μM), Fusarium culmorum (MIC=0.4-0.8 μM), Fusarium oxysporum (MIC=0.4-0.8 μM), Neurospora crassa (MIC=0.4-0.8 μM), Nectria haematococca (MIC=0.2-0.4 μM), Tricoderma viridae (MIC=0.4-0.8 μM), Tricophvton mentagrophytes (MIC=0.8-1.6 μM);
      • Yeasts: Candida albicans (MIC=0.15-0.3 μM), Candida glabrata (MIC=12.5-25 μM), Candida tropicalis (MIC=3.15-6.25 μM), Cryptococcus neoformans (MIC=0.8-1.6 μM), Saccharomyces cerevisiae (MIC=1.6-3.15 μM).
    • Hemolytic activity

      • [Ref.10942757]16% hemolytic activity at 1 μM and 100 μM against human erythrocytes
    • Binding Target

    • Unknown
    • Structure

    • Beta sheet
    • Structure Description

    • Unknownn
    • PDB ID

    • 1KFP resolved by NMR.
    • DRAMP18495 helical wheel diagram
    • 1KFP-> 
    • Formula

    • C93H158N36O24S4
    • Absent Amino Acids

    • ADEFHIMNPSW
    • Common Amino Acids

    • R
    • Mass

    • 259615
    • PI

    • 10.07
    • Basic Residues

    • 6
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 2
    • Boman Index

    • -7906
    • Hydrophobicity

    • -106.11
    • Aliphatic Index

    • 37.78
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 3230
    • Absorbance 280nm

    • 190
    • Polar Residues

    • 8

DRAMP18495

DRAMP18495 chydropathy plot
    • Function

    • Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. It shows hemolytic activity.
    • Tissue specificity

    • In hemocytes only, but not in all hemocytes observed.
  • Literature 1
    • Reference

    • J Biol Chem. 2000 Oct 27;275(43):33464-70.
    • Author

    • Silva PI Jr, Daffre S, Bulet P.
    • Title

    • Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family.