General Information

    • DRAMP ID

    • DRAMP18508

    • Peptide Name

    • gp41w-FKA (gp41 peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from the HIV glycoprotein, gp41

    • Gene

    • Not found

    • Sequence

    • KFAKKFKWFAKAAFKFFKK

    • Sequence Length

    • 19

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23019445]Gram-positive bacterium: Staphylococcus aureus (MIC<10 μg/ml, MBC=20-30 μg/ml);
      • Gram-negative bacterium: Escherichia coli (MIC=10-30 μg/ml, MBC=40-50 μg/ml)

    • Hemolytic Activity

      • [Ref.23019445]EC50=195 μg/mL against human red blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • gp41w-FKA binds at the interfacial region of a phospholipid bilayer.

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • gp41w-FKA peptide to adopt a helical conformation in the cosolvent mixture of chloroform, methanol and water. The peptide formed a very well defined

    • Helical Wheel Diagram

    • DRAMP18508 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18508.

Physicochemical Information

    • Formula

    • C125H182N28O20

    • Absent Amino Acids

    • CDEGHILMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 2397

    • PI

    • 10.85

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +8

    • Boman Index

    • -1695

    • Hydrophobicity

    • -0.426

    • Aliphatic Index

    • 21.05

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 305.56

    • Polar Residues

    • 0

DRAMP18508

DRAMP18508 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Design of a novel tryptophan-rich membrane-active antimicrobial peptide from the membrane-proximal region of the HIV glycoprotein, gp41.

    • Reference

    • Beilstein J Org Chem. 2012;8:1172-84. Epub 2012 Jul 24.

    • Author

    • Haney EF, Nguyen LT, Schibli DJ, Vogel HJ.